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EC number: 267-051-0
CAS number: 67774-74-7
The effects of LAB on zebra fish (Danio
rerio) was studied in a flow-through toxicity test. The study was
conducted according to OECD TG 203. It was not compliance with GLP but
appears to be conducted similar to GLP studies. Two groups of ten zebra
fish were exposed in an extended acute test to a test solution of LAB
for 21 days. This high exposure was obtained by using acetone as a
vehicle. The measured test concentration was 57.78 µg/L, which was above
the water solubility of 37.2 µg/L (determined in a water solubility test
conducted concurrently). No mortality, behavioural changes, or toxic
symptoms were noted in the exposure groups.
Based on the findings, the 21 -day
NOEC for zebra fish was determined to be
≥ 57.78 µg/L, the LC0 was above the water solubility
limit of LAB.
A 21-day long-term toxicity test of the test
subtsance to freshwater fish (Danio
rerio) was conducted according to OECD guideline No.203. The
NOEC based on behaviour, toxic symptoms and mortality was determinded to
be > 57.78 µg/L.
An OECD 203 study with zebra fish (Danio
rerio) was conducted in which the exposure period was extended to 21
days. Fish were exposed to LAB in a flow-through system at a limit
concentration of 58 µg/L using acetone as a solvent. Fish were fed with
Nutrafin once every three days during the test. No mortality was
observed in the treatment solutions during the study. No toxic symptoms
were observed. The 21-day NOEC is therefore greater than the limit dose
of 58 µg/L.
The lack of fish toxicity is
consistent with expectations. Overall, LAB has very low water solubility
and in a 14-day study with fish there were no effects at the limits of
water solubility. In fact, in short-term tests conducted by Gledhill et
al. (1991), fish were unaffected even at nominal concentrations of 1000
mg/L and with LAB visibly floating on the surface of the test vessels.
Furthermore, short-term studies conducted with aquatic invertebrates and
algae indicated no doseresponsive effects at the water solubility limit.
Finally, a 21-day long-term study in Daphnia was conducted and resulted
in a LOAEC of 0.015 mg/L (15 µg/L). Based on the generally greater
sensitivity of Daphnia than fish, the lack of bioaccumulation potential,
the available fish, invertebrate and algae data, and in accordance with
Annex IX, Section 9, column 2 of the REACH regulation, a long-term
toxicity study in fish is not deemed necessary.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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