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EC number: 267-051-0
CAS number: 67774-74-7
Table 1. An overview of the results.
(Treatment Mean ± Std. Dev.)
(Mean ± Std. Dev.)
Dry Weight(Mean ± Std. Dev.)
270 ± 23
4.8 ± 0.13
1.11 ± 0.147
281 ± 31
4.6 ± 0.20
1.13 ± 0.0917
306 ± 20
4.8 ± 0.21
1.19 ± 0.0958
274 ± 22
4.6 ± 0.095
1.11 ± 0.131
303 ± 21
4.8 ± 0.12
0.99 ± 0.184
This study determined the long-term
toxicity of Benzene 10 -13, alkyl derivatives to the freshwater aquatic
invertebrates, Daphnia magna. This study was conducted according
to OECD TG 211 and compliance with GLP. The daphnia were exposed to the
test substance in a semi-static system and at concentrations of 0.01,
0.1, 1.0, 10.0 mg/L for 21 days. Both immobilization and reproductive
parameters were monitored. Due to the low solubility of the test
substance, no test substance was detected in the analytical monitoring.
Thus, the results are therefore reported as a water accommodated
fraction (WAF). After 21 days exposure, no statistically significant (p
> 0.05) effect were observed on the mortality, neonate production, dry
weight or body length of the tested daphnia.
Based on the findings, the 21-day EL50
was determined to be > 10 mg/L (WAF) for both immobilization and
reproduction, the LOEL was determined to be > 10 mg/L (WAF) and the
21-day NOEL was determined to be ≥ 10 mg/L (WAF).
All available data was assessed. The
lowest effect level (NOEC = 0.0075 mg/L) was found in the 21-day chronic
study on Daphnia magna (Gledhill et al.,1991). However, in this
solubility of LAB was artificially enhanced by using acetone as a
solvent. In accordance with the OECD Guidance Document on Aquatic
Toxicity Testing of Difficult Substances and Mixtures, ENV/JM/MONO
(2000)6 and ECHA Guidance on information requirements and chemical
safety assessment (version 4.0, June 2017), Chapter R7b, Table
R.7.8-3, aquatic toxicity testing of a UVCB substance which is only
partially soluble in water should preferably be conducted using a
water-accommodated fraction (WAF) of the UVCB and solvents should be
avoided to prevent unrealistic conclusions. In addition, the
solvent concentration (1 mg/L) in this study was above the recommended
level for solvent addition (< 0.1 mL/L) (OECD Guideline
Based on these reasons,
the NOEC value from this study was considered not reliable
for the derivation of PNECs and probably overestimake the actual effect.
In comparsion, the 21-day long-term toxicity
test on freshwater invertebrate (Daphnia magna) used the
water accommodated fraction method according to OECD guideline
No.211. It was considered to be the key study and selected for CSA. The
other studies were included as supporting information. The NOEL was
determined to be ≥ 10 mg/L.
The 21-day study examined the effects
of chronic exposure of Daphnia magna to the test substance by
using the water accommodated fraction (WAF) method (OECD Guideline 211).
Ten first-instar Daphnia (< 24 h old) were individually placed in test
chambers with test solution (200 mL) containing 0, 0.01, 0.1, 1.0 or
10.0 mg/L (WAF) of the test substance. Test solutions were changed every
2-3 days. Effect parameters measured included mortality, onset of
reproduction (measured three times per week) signs of toxicity, body
length and dry weight. Due to the low water solubility of the test
substance, no test substance was detected in the analytical monitoring
(SPME-GC-FID). The results are therefore reported as a WAF (nominal
concentration). The 21-day EL50 for Daphnia magna exposed to LAB was >10
mg/L (WAF) for both immobilization and reproduction. The 21-day NOEL for
Daphnia magna was 10 mg/L (WAF) and the 21-day LOEL was >10 mg/L (WAF).
Based on these results LAB is not classified for chronic aquatic
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