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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Toxicological information

Genetic toxicity: in vitro

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Administrative data

Endpoint:
in vitro gene mutation study in bacteria
Remarks:
Type of genotoxicity: gene mutation
Type of information:
experimental study
Adequacy of study:
key study
Study period:
October - December, 2010
Reliability:
1 (reliable without restriction)

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2011
Report date:
2003

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to guideline
Guideline:
OECD Guideline 471 (Bacterial Reverse Mutation Assay)
Qualifier:
according to guideline
Guideline:
EPA OPPTS 870.5100 - Bacterial Reverse Mutation Test (August 1998)
Qualifier:
according to guideline
Guideline:
EU Method B.13/14 (Mutagenicity - Reverse Mutation Test Using Bacteria)
GLP compliance:
yes (incl. QA statement)
Type of assay:
bacterial reverse mutation assay

Test material

Constituent 1
Chemical structure
Reference substance name:
1,2-dichloropropane
EC Number:
201-152-2
EC Name:
1,2-dichloropropane
Cas Number:
78-87-5
Molecular formula:
C3H6Cl2
IUPAC Name:
1,2-dichloropropane
Details on test material:
Name. 1,2-dichloropropane
Storage: room temperature
Purity: 99.59%
Description: clear liquid

Method

Species / strainopen allclose all
Species / strain / cell type:
S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
Species / strain / cell type:
E. coli WP2 uvr A
Metabolic activation:
with and without
Metabolic activation system:
S9 mix
Test concentrations with justification for top dose:
in TA98 at 5000 and 1581 μg/plate (-S9 Mix); 5000 μg/plate (+S9 Mix)
in TA100 at 5000 and 1581 μg/plate (-S9 Mix); 5000 μg/plate (+S9 Mix)
in TA1535 at 5000 and 1581 μg/plate (-S9 Mix); 5000 μg/plate (+S9 Mix)
in TA1537 at 5000 and 1581 μg/plate (-S9 Mix); 5000 μg/plate (+S9 Mix)
in E. coli WP2 uvrA at 5000 μg/plate (±S9 Mix)
Vehicle / solvent:
Dimethyl sulfoxide (DMSO) and distilled water.
Controls
Untreated negative controls:
yes
Remarks:
Dimethyl sulfoxide (DMSO); Distilled water:
Positive controls:
yes
Remarks:
4-nitro-1,2-phenylene-diamine ; Sodium azide; 9-aminoacridine; Methyl-methanesulfonate; 2-aminoanthracene

Results and discussion

Test resultsopen allclose all
Species / strain:
S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
Metabolic activation:
with and without
Genotoxicity:
negative
Cytotoxicity / choice of top concentrations:
no cytotoxicity
Species / strain:
E. coli WP2 uvr A
Metabolic activation:
with and without
Genotoxicity:
negative
Cytotoxicity / choice of top concentrations:
no cytotoxicity
Remarks on result:
other: all strains/cell types tested
Remarks:
Migrated from field 'Test system'.

Applicant's summary and conclusion

Conclusions:
Interpretation of results (migrated information):
negative with and without metabolic activation

The test item 1,2-Dichloropropane had no mutagenic activity on the growth of the applied bacterium tester strains under the test conditions used in this study.