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EC number: 203-865-4
CAS number: 111-40-0
Bacterial mutation assays
Several reports were identified using reverse bacterial mutation assays
to determine the mutagenicity of diethylene triamine, only three gave
positive results where one of the strains tested had an increase number
of revertants. In the key guidelines study by BASF. DETA
led to an increased number of revertants using E. coli WP2 uvrA with and
without S9 using the criteria of 2 times the background for a positive
result. Negative reponses were achieved for each of
the other strains (TA98, TA100, TA1535, and TA1537). Another,
Ames test was conducted by Haskell laboratories where DETA was added to
the plate at 3,000 ug/plate. Negative results were
recorded for TA100, TA1535, TA1537 and TA1538, but were positive for
strain TA98. The test was re-ran after the preparation
of DETA used for the analysis was further purified. The
purified DETA preparation lacked any mutagenic activity in the TA 98
Another Ames test was conducted using a sample of 99.5%. In
this case, the Ames test was negative in TA1535 and TA1538, but positive
in TA98, TA100, and TA1537. The results were not
reproducible on a retest for strains TA98 and TA100. Only TA1537
showed a reproducible positive effect.
The other four reports using bacterial reverse mutation assays were
negative in the following strains: TA98, TA100, TA1535, TA1537, and
In vitro Chromosome aberration test
Diethylenetriamine (DETA) was evaluated in an in vitro chromosomal
aberration assay using Chinese hamster ovary (CHO) cells. The
concentrations of the test material were up to and including 2500
ul/plate. There were no statistical significant
increases in the frequencies of chromosomal aberrations in cultures
treated with any of the grades of DETA with or without metabolic
In vitro Mammalian cell mutation assays
All grades of DETA were negative in the CHO HPGRT, CHO Mutation, Sister
Chormatoid Exchange (SCE) , and Unscheduled DNA Synthesis assays with
and without metabolic activation. The UDS data indicated that none of
the grades of DETA over a 1000 fold conscentration range induced DNA
synthesis in this test. Moreover, none of the grades of DETA produced
dose-related increased in the frequency of SCE in tests both with and
without the incorporation of an S9 metabolic activation system. Another
report investigated a 98.9% purified grade of DETA for genotxoic
protential in mammalian cells using SCE in CHO cells. This study
indicated that DETA statistically increased the incidence of SCE without
metabolic activation. A marginal increase was observed when DETA was
tested in the presence of S9 mix.
In vivo: Drosophila Sex-Linked Recessive Lethal Test
The mutagenic potential of diethylenetriamine (DETA) was evaluated in
the Drosophila sex-linked recessive lethal (SLRL) assay. The test
chemical was administered to Drosophila melanogaster males by adult
feeding at an exposure concentration of 60 mM. DETA was considered
negative in the Drosophila SLRL assay.
In vivo: Micronucleus test
DETA was evaluated in the mouse micronucleus tests (OECD 474). It
was concluded that DETA did not significantly increase the frequency of
micronucleated polychromatic erythrocytes in the treated groups.
In vivo: Transgenic Rodent Asssay
A higher tier Transgenic Rodent Mutation assay was performed as a
definitive test to determine the mutagenic potential of DETA. Big Blue
C57B/6 mice were dosed at 50, 150, and 400 mg/kg/day via gavage for 28
days. Bone marrow, liver, and stomach tissues were taken post-exposure
and were processed for DNA isolation. The evaluation of mutation
frequencies at the Cll gene in liver, bone marrow, and stomach were
comparable to the controls. Under the conditions of the test DETA did
not demostrate any mutagenic activity.
Evidence from in vitro studies and the three in vivo tests indicate that
DETA is a non-mutagenic substance.
Short description of key information:
In vitro: Diethylene triamine was studied for its mutagenic
potential in 7 different Ames tests. Other studies include one CHO
Sister Chromatid Exchange assay, one chromosomal aberration test with
CHO, three CHO HPGRT forward mutation assays (CHO Mutation test, the
Sister Chromatid Exchange (SCE ) test and Unscheduled DNA systhesis
(UDS), and three studies examined the effects of DETA on unscheduled DNA
synthesis. Four studies were found using chinese hamster ovary cells to
determine the potential of DETA to cause chromosomal aberration.
In vivo: Two key in vivo studies were identified for determining the
mutagenic potential of diethylene triamine: a mouse micronucleus assay
and a Drosophila sex-linked recessive lethal test.
Endpoint Conclusion: No adverse effect observed (negative)
Evidence from in vitro and in vivo studies indicate that DETA in not
genotoxic and not classifiable under GHS.
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