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EC number: 203-865-4
CAS number: 111-40-0
An 8-week earthworm reproduction study was available for review.
After 28 days of exposure in soil, no earthworm mortalities were
observed in all treatments. In the lowest test item concentration of
62.5 mg test item/kg DW, two worms showed segmentations, which also
resulted in a significantly reduced average body weight at this
concentration. However, this observation was considered a biological
variation and not taken into account for the determination of the NOEC
and LOEC. In all remaining test item concentrations, no pathological
symptoms or changes in the behaviour of adult earthworms and no
statistically significant differences in growth (earthworm body weight
gain) were observed. After a further four weeks, the reproduction rate
(average number of juveniles produced per replicate) was 135 in the
control and ranged from 42 to 139 in the test item treatment groups,
with the size of the reduction increasing in response to dose. Compared
to the control, a statistically significant reduction of reproduction
was observed in the test item concentration 1000 mg test item/kg soil
dry weight. Consequently, the NOEC of the test item concerning
reproduction was determined to be 500 mg test item/kg soil dry weight.
The ECx-values in terms of mortality and growth could not be calculated,
since no effects were observed. The EC50-value for reproduction was
determined to be 797 mg/kg soil dry weight. The EC20- and EC10-values
were determined to be 537 mg test item/kg soil dry weight and 437 mg
test item/kg soil dry weight, respectively.
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