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EC number: 203-002-1
CAS number: 102-06-7
potential for 1,3-diphenylguanidine to induce chromosomal aberrations in
the bone marrow cells of Sprague-Dawley rats was tested. Based on
results from the toxicity rangefinding experiments,
1,3-Diphenylguanidine (DPG) was administered via oral gavage to male and
female rats at a target dose of 300 mg/kg body weight (approximately 70%
of the combined LD50). Control groups received 10 ml/kg of body weight
of vehicle control (corn oil) or a 40 mg/kg of body weight dose of
positive control (cyclophosphamide). Bone marrow was sampled at 6, 24
and 48 hours alter dosing with the vehicle or DPG. A single sampling
time of 24 hours alter dosing was used for the cyclophosphamide control
group. Slides were scored for increases in the proportion of aberrant
metaphases and in the frequency of aberrations/cell.
the main cytogenetic experiment, DPG was toxic to male and female rats
as evidenced by clinical signs of toxicity (hypoactive and
nonresponsive) and death. Five male rats and six female rats were found
dead within 24 hours of dosing. Statistically significant decreases in
mean body weight were observed in the DPG treated male and female rats
at 6 and 24 hours alter treatment and in the positive control treated
male rats 24 hours afier treatment.
statistically significant increases in the proportion of aberrant cells
or aberrations/cell were observed at the 6, 24 and 48 hour time points.
Significant induction of toxicity, measured as mitotic index depression.
was observed at the 6 hour (35%) and 24 hour (31%) time points. No
depression in mitotic index was observed at the 48-hour time point.
positive control group (cyclophosphamide) yielded expected positive
responses indicating the adequacy of our experimental conditions for the
detection of clastogens.
observations and findings of this study indicate that
1,3-diphenylguanidine was non clastogenic. It did not induce increases
in the proportion of aberrant cells or aberrations/cell under the
experimental conditions utilized in this study.
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