Registration Dossier
Registration Dossier
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 203-002-1 | CAS number: 102-06-7
Long-term toxicity to fish
Administrative data
Link to relevant study record(s)
Description of key information
A long-term study (34 days) was conducted on Pimephales promelas eggs (OECD 210 guidelines, semi-static method). NOEC for both hatching and larval survival rates were 1.3 mg/L
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 1.3 mg/L
Additional information
DPG (1,3-Diphenylguanidine) has been shown to be toxic to fish and algae and harmful to daphnia in several acute studies (fish : 96 h LC50 = 4.2-11 mg/l; algae : 72 h EC50 = 7.5 mg/l; 96 h EC50 = 1.7 mg/l; daphnid : 24 h EC50 = 73.6 mg/l; 48 h EC50 = 17mg/l). The PNEC can be determined using the NOECs from the algae (0.3 mg/l) and daphnid chronic 21 d (0.6 mg/l) studies (excluding the EbC50 results), by applying an uncertainty factor of 50. The resulting PNEC would be 6 µg/l. In order to refine this PNEC value for the aquatic compartment we performed a long-term test on fish (following OECD 210 guidelines).
In brief, Pimephales promelas eggs were exposed to graded concentrations of DPG for 34 days (0.041, 0.13, 0.41, 1.3 and 4.2 mg/L) under semi-static conditions. After 8 days, all larvae had hatched. Both hatching and larval survival rates were recorded daily. At the end of the test, all surviving larvae were weighted and measured. No significant effect were observed on fish length and weight. The NOEC for both hatching and larval survival was 1.3 mg/L. EC50 for larval survival was 1.8 mg/L.
From this result, the retained NOEC will be 0.3 mg/L (algae results).
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
