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EC number: 203-002-1
CAS number: 102-06-7
A phytotoxicity test was performed to determine the effect of 1,3-diphenylguanidine on the freshwater alga Selenastrum capricornutum. Results of the test are reported as 24-, 48-, 72-, and 96-hour EC50's (the concentrations of the test material estimated to cause a 50% decrease of in vivo chlorophyllian exposed cultures as compared to the control at the specified times). Cell numbers in exposed and control cultures were also determined after 96 hours of exposure and another 96-hour EC50 was calculated (the concentration of the test material estimated to cause a 50% decrease of cell numbers in exposed cultures as compared to the control). This study on the toxicity of 1,3 -diphenylguanidine to algae permitted to estimate a EC50 -96h = 1.4 - 1.7 mg/l concerning the cell division rate (determined by cell counter) and an NOEC at 0.3 mg/l
A phytotoxicity test was performed at Bionomics Marine
Research Laboratory (BMRL), Pensacola, Florida, to determine the effect
of DPG (BN-79-1384358-le) on the freshwater alga Selenastrum
capricornutum. Results of the test are reported as 24-,
48-, 72-, and 96-hour EC50. Cell numbers in exposed and control cultures
were also determined after 96 hours of exposure and another 96-hour EC50
This study on the toxicity of 1,3 -diphenylguanidine to
algae permitted to estimate an EC50 -96h = 1.4 - 1.7 mg/l concerning the
cell division rate (determined by cell counter). The results obtained
during this test are summarized hereafter:
EC50s based on chlorophyll a determination:
24h >5.6 mg/l
48h 3.5 mg/l (0.2-56)
72h 2.0 mg/l (0.2-17)
96h 1.4 mg/l (0.2-7.4)
96h EC50 based on cell number = 1.7 mg/l (0.4-7.4)
NOEC not calculated but based on available data can be taken
as 0.3 mg/l at both 72 and 96h (for chlorophyll a and growth
rate data at 96h).
In another test relative to the cell division inhibition
(DIN 38412 part 9) the following values were determined for the green
algae Scenedesmus subspicatus after 72 hours at a pH of 7.6 - 8.9
and 23°C+/-2°C: EC10 = 0.013 mg/l and EC50 = 2.6 mg/l (based on the
biomass); EC10 = 2.1 mg/l and EC50 = 7.5 mg/l (based on the growth rate).
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