Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 215-572-9
CAS number: 1332-65-6
Table 2a. Summary
1: 12-14 hour sacrifice time.
Net Nuclear Grain Count
Net Grain Count of Cells in Repair
Percent of Cells in Repair (Net Grain Count ≥5)
Copper II sulphate pentahydrate
Table 2b. Summary
Experiment 2: 2-4 hour sacrifice time.
sulphate pentahydrate was tested for its ability to induce unscheduled
DNA synthesis (UDS) in the livers of orally dosed male rats using an in
vivo/in vitro procedure. Groups
of 6 male rats were treated once with copper sulphate at 632.5 or 2000
mg/kg by oral gavage at a dose volume of 10 ml/kg. For
the negative control, a further 6 male rats received purified water as a
negative control at the same dose volume. Positive
control animals for the 12-14 hour experiment, 6 male rats were dosed
orally with 75 mg/kg 2 -acetamidofluorene, suspended in corn oil.
dissolved in purified water, was the positive control for the 2-4 hour
12-14 hours (experiment 1) or 2-4 hours (experiment 2) after dose
administration the animals were sacrificed and the livers perfused with
collagenase to provide a primary culture of hepatocytes. The
net grain count, number of grains present in the nucleus, minus the mean
number of grains in 3 equivalent areas of cytoplasm were determined.
animals gave a group mean net grain of less than 0 with no cells in
mean net grain values were increased by both positive controls to more
than 5 with more than 50% of cells found to be in repair. This
was consistent with historical control data.
632.5 or 2000mg/kg copper sulphate pentahydrate (equivalent to 161 or
509 mg Cu/kg) did not produce a group mean net grain value greater than
-1.0 nor were any more than 1.0% cells found in repair at either dose.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
Welcome to the ECHA website. This site is not fully supported in Internet Explorer 7 (and earlier versions). Please upgrade your Internet Explorer to a newer version.
Do not show this message again