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EC number: 215-572-9
CAS number: 1332-65-6
Table 1. Summary of Mortalities
Time of death
Day 3; Day 4;Day 5 (2); Day 7
Day 2; Day 3 (2)
Day 1; Day 2 (3); Day 3
Day 1; Day 2;Day 4 (2)
Figures in parenthesis are the number which died on the day specified if more than one.
A GLP-compliant acute oral toxicity study was conducted in accordance
with the requirements of EU Guideline B.1 and OECD 401 without
significant deviation. Dicopper chloride trihydroxide
was administered as a suspension in Alembicol D. Groups of five male
and five female Hsd:ICR(CD-1) mice weighing 26 to 33 g (males) and 23 to
28 g (females) were used. The mice were acclimatised, housed in groups
of up to five by sex and fasted overnight prior to dosing. Food was
returned immediately after dosing. Dose levels (based on a
range-finding study) of 200, 400 and 800 mg/kg bw were administered by
single oral administration by metal cannula at 10 mL/kg on Day 1.
Animals were observed frequently on the day of dosing and then daily for
the 14‑day post-dosing period. Animals were weighed prior to
administration and after 7 days (on Day 8) and after 14 days (on Day 15)
or at death. Decedents and animals surviving to 14 days were subject to
There was one male and one female mortality at 200 mg/kg bw. At
400 mg/kg bw all males and three females died, and at 800 mg/kg bw all
males and four females died. The deaths occurred between Day 1 and Day
7. There was a variety of clinical signs recorded including reduced
activity, salivation, partly-closed eyes, matted fur, staining of
skin/fur in the urogenital region, liquid faeces, hunched posture,
pallor, lethargy and unconsciousness. Males treated at 400 and
800 mg/kg bw, and females treated at 800 mg/kg bw, produced faeces
coloured blue, thought to be unabsorbed test substance. Some of the
symptoms became apparent on the day of treatment and others on Day 2 or
later. Surviving animals recovered within the first week after
treatment. All surviving animals showed expected weight gain during the
study. No gross findings were recorded in surviving animals at
necropsy. The most notable necropsy finding in animals that died during
the study was green coloured material in the digestive tract. Staining
of the skin/fur particularly the urogenital areas was also recorded in
most animals. The acute oral LD50 (calculated by probit analysis) of
dicopper chloride trihydroxide to the mouse was 299 mg/kg bw for the
sexes combined (with 95% confidence limites of 215 to 414 mg/kg bw).
This study has been
disregarded for classification and labelling purposes as the mouse is
not a preferred species under REACH.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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