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EC number: 215-572-9
CAS number: 1332-65-6
Table 1. Summary of Mortalities.
Time of death
Day 6 (2)
Day 5 (2);Day 6; Day 7
Day 3 (2); Day 5; Day 6; Day 7
Day 3 (2); Day 6
Day 4 (2);Day 5 (2); Day 6
Day 2; Day 3;Day 7
Figures in parenthesis are the number which died on the day specified if more than one.
A GLP-compliant acute oral toxicity study was conducted in accordance
with the requirements of EU Guideline B.1 and OECD 401. Dicopper
chloride trihydroxide was administered as a solution in carboxymethyl
cellulose. Groups of five male and five female Sprague-Dawley rats
weighing 127 to 204 g were used. The rats were housed in groups of five
by sex, acclimatised, and fasted overnight prior to dosing. Food was
returned four hours after dosing. Dose levels (based on a range-finding
study) of 1000, 1400, 1800 and 2200 mg/kg bw were administered by single
oral administration by gavage in 10 mL/kg on Day 1. Animals were
observed frequently on the day of dosing and then once daily for the
14‑day post-dosing period. Animals were weighed prior to administration
and after 7 days (on Day 8) and after 14 days (on Day 15) or at
death. Decedents and animals surviving to 14 days were subject to gross
There were two male mortalities but no female mortalities at 1000 mg/kg
bw. At higher doses, the frequency of mortality increased and all males
and three females died (or were killed in extremis) following 1800 and
2200 mg/kg bw. The deaths occurred between Day 2 and Day 7. There was a
variety of clinical signs recorded including piloerection, nasal
discharge, ataxia, subdued behaviour, diarrhoea, hunched and pale
appearance, laboured or slow breathing, swollen abdomen, tail lesions,
soiled coats with anal and perigenital staining, ocular discharge,
dull/opaque eyes, vocalisation, hypothermia, alopecia and tremors. Most
symptoms occurred between Day 2 and 7 though some effects persisted up
to Day 15. Surviving animals showed weight gain during the study. No
gross findings were recorded in surviving animals at necropsy. The most
notable necropsy finding in animals which died during the study was a
green coloured, clear or dark liquid in the digestive tract. Foci,
thickened mucosa and mucoid or liquid contents were also found in the
caecum and one male treated at 2200 mg/kg bw had pale lungs.
The acute oral LD50 (calculated by probit analysis) of Dicopper chloride
trihydroxide to the rat was 1083 mg/kg bw for males (with 95% confidence
limits of 239 to 1355 mg/kg bw), 1854 mg/kg bw for females (with 95%
confidence limits of 1398 to 4909 mg/kg bw) and 1398 mg/kg bw for the
sexes combined (with 95% confidence limits of 1018 to 1706 mg/kg bw). On
this basis, dicopper chloride trihydroxide is classified as Acute Tox.
4, H302: Harmful if swallowed.
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