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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Toxicological information

Genetic toxicity: in vitro

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Administrative data

Endpoint:
in vitro gene mutation study in mammalian cells
Remarks:
Type of genotoxicity: gene mutation
Type of information:
experimental study
Adequacy of study:
supporting study
Reliability:
4 (not assignable)
Rationale for reliability incl. deficiencies:
other: Only short abstract available

Data source

Reference
Reference Type:
publication
Title:
Genotoxicity of three acrylate compounds in L5178Y mouse lymphoma cells.
Author:
Amtower AL, Brock KH, Doerr CL, Dearfield KL, Moore MM
Year:
1986
Bibliographic source:
Envir. Molec. Mutagen. 8 (6): 4

Materials and methods

Principles of method if other than guideline:
L5178Y Mouse lymphoma TK Assay
GLP compliance:
not specified
Type of assay:
mammalian cell gene mutation assay

Test material

Constituent 1
Chemical structure
Reference substance name:
Methyl acrylate
EC Number:
202-500-6
EC Name:
Methyl acrylate
Cas Number:
96-33-3
Molecular formula:
C4H6O2
IUPAC Name:
methyl acrylate
Details on test material:
no further data

Method

Target gene:
TK+/-
Species / strain
Species / strain / cell type:
mouse lymphoma L5178Y cells
Metabolic activation:
without
Metabolic activation system:
S-9 (unspecified)
Test concentrations with justification for top dose:
22 µg/mL; other doses not stated
Details on test system and experimental conditions:
DURATION
- Exposure duration: 4 h

Results and discussion

Test results
Species / strain:
mouse lymphoma L5178Y cells
Metabolic activation:
without
Genotoxicity:
positive
Cytotoxicity / choice of top concentrations:
cytotoxicity
Vehicle controls validity:
not specified
Untreated negative controls validity:
not valid
Positive controls validity:
not specified
Additional information on results:
Methyl acrylate was mutagenic (~440 mutants/10E+06 survivors) at 22 µg/mL; a dose that gave > 10 % survival.
Remarks on result:
other: all strains/cell types tested
Remarks:
Migrated from field 'Test system'.

Any other information on results incl. tables

The TS induced primarily small-colony, TFT-resistant mutants which predict that the compound is clastogenic.

Applicant's summary and conclusion