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Toxicological information

Genetic toxicity: in vitro

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Administrative data

Endpoint:
in vitro gene mutation study in mammalian cells
Remarks:
Type of genotoxicity: gene mutation
Type of information:
experimental study
Adequacy of study:
supporting study
Reliability:
4 (not assignable)
Rationale for reliability incl. deficiencies:
other: Only short abstract available

Data source

Reference
Reference Type:
publication
Title:
Genotoxicity of three acrylate compounds in L5178Y mouse lymphoma cells.
Author:
Amtower AL, Brock KH, Doerr CL, Dearfield KL, Moore MM
Year:
1986
Bibliographic source:
Envir. Molec. Mutagen. 8 (6): 4

Materials and methods

Principles of method if other than guideline:
L5178Y Mouse lymphoma TK Assay
GLP compliance:
not specified
Type of assay:
mammalian cell gene mutation assay

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
no further data

Method

Target gene:
TK+/-
Species / strain
Species / strain / cell type:
mouse lymphoma L5178Y cells
Metabolic activation:
without
Metabolic activation system:
S-9 (unspecified)
Test concentrations with justification for top dose:
22 µg/mL; other doses not stated
Details on test system and experimental conditions:
DURATION
- Exposure duration: 4 h

Results and discussion

Test results
Species / strain:
mouse lymphoma L5178Y cells
Metabolic activation:
without
Genotoxicity:
positive
Cytotoxicity / choice of top concentrations:
cytotoxicity
Vehicle controls validity:
not specified
Untreated negative controls validity:
not valid
Positive controls validity:
not specified
Additional information on results:
Methyl acrylate was mutagenic (~440 mutants/10E+06 survivors) at 22 µg/mL; a dose that gave > 10 % survival.
Remarks on result:
other: all strains/cell types tested
Remarks:
Migrated from field 'Test system'.

Any other information on results incl. tables

The TS induced primarily small-colony, TFT-resistant mutants which predict that the compound is clastogenic.

Applicant's summary and conclusion