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Toxicological information

Acute Toxicity: inhalation

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Administrative data

Endpoint:
acute toxicity: inhalation
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: Comparable to guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1980
Report Date:
1980

Materials and methods

Test guideline
Qualifier:
equivalent or similar to
Guideline:
OECD Guideline 403 (Acute Inhalation Toxicity)
Deviations:
no
Principles of method if other than guideline:
This test was performed in principle as described in OECD Guideline 403.
Ten male and ten female rats (Sprague-Dawley) were exposed by head-nose exposure to butyl acrylate vapour analyzed concentrations of 2.7, 3.6, 4.96, 6.8, 8.1, 12.1 and 16.0 mg/L. The animals were observed over a 14 day period after exposure.
GLP compliance:
no
Test type:
standard acute method
Limit test:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent
Test material form:
other: liquid

Test animals

Species:
rat
Strain:
Sprague-Dawley
Sex:
male/female
Details on test animals and environmental conditions:
TEST ANIMALS
- Source: SPRA (SPF 68 Han), MUS RATTUS, Brunnthal
- Weight at study initiation: 185±15 g
- Diet: Herilan MRH, H . EGGERSMANN KG, ad libitum
- Water: Tap water, ad libitum



Administration / exposure

Route of administration:
inhalation: vapour
Type of inhalation exposure:
nose/head only
Remarks:
head-nose exposure
Vehicle:
other: unchanged (no vehicle)
Details on inhalation exposure:
GENERATION OF TEST ATMOSPHERE / CHAMBER DESCRIPTION
A dynamic inhalation system was used in the present study where exposure concentrations of butyl acrylate were generated by pumping liquid butyl acrylate at constant rates into glass vaporization flasks heated to 70°C, the vapours were mixed with fresh air and transmitted to the exposure chamber.


TEST ATMOSPHERE
- Brief description of analytical method used: The butyl acrylate air mixture was measured continuously using a flame ionization detector (FID). Apparatus used was FID total hydrocarbons analyzer (CARLO ERBA).
- Samples taken from breathing zone: yes


Analytical verification of test atmosphere concentrations:
yes
Duration of exposure:
4 h
Concentrations:
2.7, 3.6, 4.96, 6.8, 8.1, 12.1 and 16.0 mg/L
No. of animals per sex per dose:
10
Control animals:
no
Details on study design:
- Duration of observation period following administration: 14 days
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, body weight, gross pathology
Statistics:
Statistical analysis were made according to Probit-Analysis, D.J. Finney, 1971.

Results and discussion

Effect levelsopen allclose all
Sex:
male/female
Dose descriptor:
LC50
Effect level:
10.3 mg/L air (analytical)
Exp. duration:
4 h
Sex:
male
Dose descriptor:
LC50
Effect level:
11.2 mg/L air (analytical)
Exp. duration:
4 h
Sex:
female
Dose descriptor:
LC50
Effect level:
10 mg/L air (analytical)
Exp. duration:
4 h
Mortality:
No animals died in the 2.7, 3.6 and 4.96 mg/L dose groups, respectively. 1/20, 4/20, 13/20 and 20/20 died in the 6.8, 8.1, 12.1 and 16.0 mg/L dose groups, respectively.
Clinical signs:
other: No clinical symptoms were observed in the 2.7 mg/L dose group. In the 3.6 mg/L dose group, irregular respiration and squatting position were observed, as well as unregular gait. In the 4.96 to 8.1 mg/L dose groups eye and nasal secretion, noisy irregular
Body weight:
There was no adverse effect on the body weight gain in all male animals of the dose groups (6.8 and 2.7 mg/L) and all female animals of the dose groups (8.1, 6.8 and 4.96 mg/L) in comparison to controls. The body weight gain in the male animals of dose groups (12.1 and 3.6 mg/L) and in female animals of dose groups (12.1, 3.6 and 2.7 mg/L) were strongly affected 7 days after the exposure. All the animals in the affected groups recovered by the end of the experimental period except for the male animals of dose groups (12.1 and 3.6 mg/L).
Gross pathology:
Acute dilatation and acute hyperemia of the heart, spotted haemorrhages and acute emphysema in lungs, broaden grey-brown periphery of hepatic lobules were observed in the deceased animals. No pathological lesions were observed in the sacrificed animals.

Any other information on results incl. tables

Concentration (mg/L)

Mortality

Analytical

Nominal

Male

Female

16.0

17.8

10/10

10/10

12.1

12.6

7/10

6/10

8.1

8.4

0/10

4/10

6.8

7.1

1/10

0/10

4.96

5.04

0/10

0/10

3.6

3.9

0/10

0/10

2.7

2.6

0/10

0/10

1 . LC50 (4 h) for male and females rats = 10.3 mg/ L air (1970 ppm)

2 . LC50 (1 h) for male and females rats = 41.2 mg / L air (7860 ppm)*

3 . LC50 (4 h) for male rats = 11.2 mg / L air (2140 ppm)

4 . LC50 (1 h) for male rats = 44.8 mg / L air (8540 ppm)*

5 . LC50 (4 h) for female rats = 10.0 mg / L air (1910 ppm)

6 . LC50 (1 h) for female rats = 45.0 mg / L air (7630 ppm)*

* calculated from 4h LC50 values

Applicant's summary and conclusion