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Toxicological information

Basic toxicokinetics

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Administrative data

Endpoint:
basic toxicokinetics in vitro / ex vivo
Type of information:
experimental study
Adequacy of study:
key study
Study period:
25 Jan 2001 to 30 Jan 2001
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Acceptable study report which meets basic scientific principles

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2001
Report Date:
2001

Materials and methods

Objective of study:
metabolism
Principles of method if other than guideline:
Butyl acrylate was tested for relative rates of hydrolysis by porcine hepatic esterase.
GLP compliance:
yes

Test material

Reference
Name:
Unnamed
Type:
Constituent
Test material form:
other: liquid
Radiolabelling:
no

Administration / exposure

Duration and frequency of treatment / exposure:
2 min, 5 min and 3 hours
Doses / concentrations
Remarks:
Doses / Concentrations:
0.2, 0.5 and 2 mM

Results and discussion

Toxicokinetic / pharmacokinetic studies

Toxicokinetic parameters
Toxicokinetic parameters:
other: n-Butyl acrylate was rapidly hydrolyzed at rates ranging between 54-69 µmoles/min/mg protein (4 - 33 %) after 2 minutes and 43-72 µmoles/min/mg protein (10 - 66 %) after 5 minutes of incubation at 37°C.

Metabolite characterisation studies

Metabolites identified:
no

Any other information on results incl. tables

The % conversion results of n-Butyl acrylate (an average of 3 determinations)

Concentration (mM)

% Conversion / Rate*

At 2 min.

At 5 min.

2.00

4.18 / 69.67

10.09 / 67.27

0.50

18.16 / 75.67

43.72 / 72.87

0.20

32.80 / 54.67

65.97 / 43.98

* in μmoles / min / mg protein

n-Butyl acrylate was rapidly hydrolyzed at rates ranging between 54-69 µmoles/min/mg protein (4 - 33 %) after 2 minutes and 43-72 µmoles/min/mg protein (10 - 66 %) after 5 minutes of incubation at 37°C.

Applicant's summary and conclusion