Calcium iodate

Genetic toxicity: in vitro

Administrative data

Endpoint:

in vitro DNA damage and/or repair study

Remarks:

Type of genotoxicity: DNA damage and/or repair

Type of information:

migrated information: read-across based on grouping of substances (category approach)

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Data source

Reference

Reference Type:

publication

Title:

Lack of genotoxicity of potassium iodate in the alkaline comet assay and in the cytokinesis-block micronucleus test. Comparison to potassium bromate

Author:

Poul,et.al.

Year:

2004

Bibliographic source:

Food and Chemical Toxicology (2004),42(2),203-204

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other:

Principles of method if other than guideline:

Cells were exposed for 3 h to concentrations of the test compounds of 0.625, 1.25, 2.5, 5 and 10 mM. Tests compound was dissolved in the culture medium. Etoposide (0.5 µg/ ml) was dissolved in DMSO.
After cells exposure to the test compounds for 3 h, the culture medium was discarded and replaced by fresh medium. Cells were incubated for 0.5, 1 or 24 h and DNA damage was measured at each sampling time by the alkaline comet assay.

GLP compliance:

not specified

Type of assay:

comet assay

Test material

Test material information

Constituent 1

Reference substance name:

Potassium iodate

EC Number:

231-831-9

EC Name:

Potassium iodate

Cas Number:

7758-05-6

IUPAC Name:

potassium iodate

Details on test material:

- Name of test material: Potassium iodate
- Molecular formula: HIO3.K
- Molecular weight: 214.02
- Substance type: Inorganic
- Physical state: Solid
- Potassium iodate obtained from Acros organics

Method

Target gene:

no data

Species / strain

Species / strain / cell type:

Chinese hamster Ovary (CHO)

Details on mammalian cell type (if applicable):

Chinese hamster ovary (CHO-K1) cells were purchased from Eurobio (France). They were routinely maintained from stocks stored in liquid nitrogen. CHO cells were grown at 37 C in a humidified atmosphere at 5% CO2 in air, in HAM’S F12 medium with l-glutamine supplemented with 10% fetal calf serum (FCS), penicillin (50 UI/ml) and streptomycine (50 µg/ml). Cells were subcultured 24 h before treatment.

Additional strain / cell type characteristics:

not specified

Metabolic activation:

not specified

Test concentrations with justification for top dose:

0.625, 1.25, 2.5, 5 and 10 mM

Vehicle / solvent:

culture medium

Controls

Untreated negative controls:

yes

Negative solvent / vehicle controls:

not specified

True negative controls:

not specified

Positive controls:

yes

Positive control substance:

other: Etoposide (0.5 µg/ml)

Results and discussion

Test results

Species / strain:

Chinese hamster Ovary (CHO)

Metabolic activation:

not specified

Genotoxicity:

negative

Remarks:

The results showed that the comet assay failed to detect the presence of DNA damage after a treatment of cells by potassium iodate for concentrations up to 10 mM.

Cytotoxicity / choice of top concentrations:

not specified

Vehicle controls validity:

not specified

Untreated negative controls validity:

valid

Positive controls validity:

valid

Remarks on result:

other: strain/cell type: Chinese hamster ovary (CHO-K1) cells

Remarks:

Migrated from field 'Test system'.

Applicant's summary and conclusion

Conclusions:

Interpretation of results (migrated information):
negative

Potassium iodate did not induce DNA damage in CHO cells when tested at concentrations up to 10 mM in alkaline comet assay.

Executive summary:

Potassium iodate did not induce DNA damage in CHO cells when tested at concentrations up to 10 mM in alkaline comet assay.