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Toxicological information

Genetic toxicity: in vitro

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Administrative data

Endpoint:
in vitro DNA damage and/or repair study
Remarks:
Type of genotoxicity: DNA damage and/or repair
Type of information:
migrated information: read-across based on grouping of substances (category approach)
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)

Data source

Reference
Reference Type:
publication
Title:
Lack of genotoxicity of potassium iodate in the alkaline comet assay and in the cytokinesis-block micronucleus test. Comparison to potassium bromate
Author:
Poul,et.al.
Year:
2004
Bibliographic source:
Food and Chemical Toxicology (2004),42(2),203-204

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
other:
Principles of method if other than guideline:
Cells were exposed for 3 h to concentrations of the test compounds of 0.625, 1.25, 2.5, 5 and 10 mM. Tests compound was dissolved in the culture medium. Etoposide (0.5 µg/ ml) was dissolved in DMSO.
After cells exposure to the test compounds for 3 h, the culture medium was discarded and replaced by fresh medium. Cells were incubated for 0.5, 1 or 24 h and DNA damage was measured at each sampling time by the alkaline comet assay.
GLP compliance:
not specified
Type of assay:
comet assay

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Name of test material: Potassium iodate
- Molecular formula: HIO3.K
- Molecular weight: 214.02
- Substance type: Inorganic
- Physical state: Solid
- Potassium iodate obtained from Acros organics

Method

Target gene:
no data
Species / strain
Species / strain / cell type:
Chinese hamster Ovary (CHO)
Details on mammalian cell type (if applicable):
Chinese hamster ovary (CHO-K1) cells were purchased from Eurobio (France). They were routinely maintained from stocks stored in liquid nitrogen. CHO cells were grown at 37 C in a humidified atmosphere at 5% CO2 in air, in HAM’S F12 medium with l-glutamine supplemented with 10% fetal calf serum (FCS), penicillin (50 UI/ml) and streptomycine (50 µg/ml). Cells were subcultured 24 h before treatment.
Additional strain / cell type characteristics:
not specified
Metabolic activation:
not specified
Test concentrations with justification for top dose:
0.625, 1.25, 2.5, 5 and 10 mM
Vehicle / solvent:
culture medium
Controls
Untreated negative controls:
yes
Negative solvent / vehicle controls:
not specified
True negative controls:
not specified
Positive controls:
yes
Positive control substance:
other: Etoposide (0.5 µg/ml)

Results and discussion

Test results
Species / strain:
Chinese hamster Ovary (CHO)
Metabolic activation:
not specified
Genotoxicity:
negative
Remarks:
The results showed that the comet assay failed to detect the presence of DNA damage after a treatment of cells by potassium iodate for concentrations up to 10 mM.
Cytotoxicity / choice of top concentrations:
not specified
Vehicle controls validity:
not specified
Untreated negative controls validity:
valid
Positive controls validity:
valid
Remarks on result:
other: strain/cell type: Chinese hamster ovary (CHO-K1) cells
Remarks:
Migrated from field 'Test system'.

Applicant's summary and conclusion

Conclusions:
Interpretation of results (migrated information):
negative

Potassium iodate did not induce DNA damage in CHO cells when tested at concentrations up to 10 mM in alkaline comet assay.
Executive summary:

Potassium iodate did not induce DNA damage in CHO cells when tested at concentrations up to 10 mM in alkaline comet assay.