Calcium dodecylbenzenesulphonate

Toxicity to other aquatic organisms

Link to relevant study record(s)

Reference

Endpoint:

toxicity to other aquatic vertebrates

Type of information:

read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Justification for type of information:

C12 LAS; Linear Alkylbenzene Sulfonate (LAS)) is a very close analogue of Calcium dodecylbenzenesulfonate (CAS No 26264-06-2, EC Number; 247-557-8) ) and read-across is valid.

Reason / purpose for cross-reference:

read-across source

Qualifier:

no guideline followed

Principles of method if other than guideline:

Thirty mussels (5.8 cm average main axis length) collected from a mussel cultivation area of the Lagoon of Venice, Italy were divided into groups of 10 and placed in net tubes in a 60-L tank for 7 days in contact with 50 mg/L continuously suspended LAS-spiked sediments. Mussels were fed an algal suspension and water, food, and sediment were renewed daily. Filtration rate was determined twice a day (immediately before and after water changes) as defined by the volume of water cleared of algal particles/animal/hour. Faeces were collected daily and pooled for analysis of LAS concentrations by HPLC. Oxygen consumption and ammonia excretion rates were determined at the end of each treatment.

GLP compliance:

no

Analytical monitoring:

yes

Vehicle:

no

Test organisms (species):

other: Mytilus galloprovincialis

Details on test organisms:

Mytilus galloprovincialis (marine mussel).
Thirty mussels (5.8 cm average main axis length) collected from a mussel cultivation area of the Lagoon of Venice, Italy

Test type:

semi-static

Water media type:

saltwater

Limit test:

yes

Total exposure duration:

7 d

Nominal and measured concentrations:

50 mg/L continuously suspended LAS-spiked sediments

Details on test conditions:

Mussels were divided into groups of 10 and placed in net tubes in a 60-L tank for 7 days in contact with 50 mg/L continuously suspended LAS-spiked sediments. Mussels were fed an algal suspension and water, food, and sediment were renewed daily.

Reference substance (positive control):

not specified

Duration:

7 d

Dose descriptor:

NOEC

Effect conc.:

32.19 other: mg/kg dry weight (geometric mean of initial [132 mg/L] and final [7.85 mg/L] LAS concentration)

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

other: filtration rate, oxygen uptake, nitrogen excretion

Details on results:

No significant differences in survival or physiological responses between treatments and controls were observed.
NOEC = 32.19 mg/kg dry weight (geometric mean of initial [132 mg/L] and final [7.85 mg/L] LAS concentration)

The LAS concentration in treated sediments decreased by about 90% over the duration of the study (mean 132 mg/kg at initiation to mean 7.85 mg/kg at completion).

Validity criteria fulfilled:

yes

Conclusions:

NOEC = 32.19 mg/kg dry weight (geometric mean of initial [132 mg/L] and final [7.85 mg/L] LAS concentration)

Description of key information

No significant differences in survival or physiological responses between treatments and controls were observed in the study of Marin, M.G., Pivotti, L., Campesan, G., Turchetto, M. and Tallandini, L.1994.
Thirty mussels (5.8 cm average main axis length) collected from a mussel cultivation area of the Lagoon of Venice, Italy were divided into groups of 10 and placed in net tubes in a 60-L tank for 7 days in contact with 50 mg/L continuously suspended LAS-spiked sediments. Mussels were fed an algal suspension and water, food, and sediment were renewed daily. Filtration rate was determined twice a day (immediately before and after water changes) as defined by the volume of water cleared of algal particles/animal/hour. Faeces were collected daily and pooled for analysis of LAS concentrations by HPLC. Oxygen consumption and ammonia excretion rates were determined at the end of each treatment.
NOEC = 32.19 mg/kg dry weight (geometric mean of initial [132 mg/L] and final [7.85 mg/L] LAS concentration)

Additional information

No significant differences in survival or physiological responses between treatments and controls were observed in the study of Marin, M.G., Pivotti, L., Campesan, G., Turchetto, M. and Tallandini, L.1994.

Thirty mussels (5.8 cm average main axis length) collected from a mussel cultivation area of the Lagoon of Venice, Italy were divided into groups of 10 and placed in net tubes in a 60-L tank for 7 days in contact with 50 mg/L continuously suspended LAS-spiked sediments. Mussels were fed an algal suspension and water, food, and sediment were renewed daily. Filtration rate was determined twice a day (immediately before and after water changes) as defined by the volume of water cleared of algal particles/animal/hour. Faeces were collected daily and pooled for analysis of LAS concentrations by HPLC. Oxygen consumption and ammonia excretion rates were determined at the end of each treatment.

NOEC = 32.19 mg/kg dry weight (geometric mean of initial [132 mg/L] and final [7.85 mg/L] LAS concentration)