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Toxicological information

Acute Toxicity: other routes

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Administrative data

Endpoint:
acute toxicity: other routes
Type of information:
experimental study
Adequacy of study:
supporting study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: A scientifically sound study but no individual data, no necroscopy or histological data.

Data source

Reference
Reference Type:
publication
Title:
Comparative percutaneous toxicity of ten industrial solvents in the guinea pig
Author:
Wahlberg, J., E.; Boman, A.;
Year:
1979
Bibliographic source:
Scand. j. work environ. & health, vol. 5, p. 345-351

Materials and methods

Test guideline
Qualifier:
no guideline followed
Principles of method if other than guideline:
0.5 and 2.0 mL of CTC were applied via intraperitoneal injection to guinea pigs weighting 372 ± 3 g (low dose group) and 373 ± 6 g (high dose group); the mortality rate was determined after an observation period of 35 d
GLP compliance:
no
Limit test:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): carbon tetrachloride
- Physical state: not reported
- Analytical purity: p. a.
- supplier: Merck, Darmstadt, Germany
- Stability under test conditions: not reported
- Storage condition of test material: not reported

Test animals

Species:
guinea pig
Strain:
not specified
Sex:
not specified
Details on test animals and environmental conditions:
TEST ANIMALS
- Source: not reported
- Age at study initiation: not reported
- Weight at study initiation: 372 ± 3 g low dose group, 373 ± 5 g high dose group
- Fasting period before study: not reported
- Housing: in groups of three in Makrolon cages
- Diet (e.g. ad libitum): standard laboratory animal food (Astra Ewos, Sodcttalje, Sweden), ad libitum
- Water (e.g. ad libitum): water supplemented with ascorbic acid, ad libitum
- Acclimation period: not reported


ENVIRONMENTAL CONDITIONS
- Temperature (°C): not reported
- Humidity (%): not reported
- Air changes (per hr): not reported
- Photoperiod (hrs dark / hrs light): not reported

Administration / exposure

Route of administration:
intraperitoneal
Vehicle:
unchanged (no vehicle)
Details on exposure:
0.5 and 2.0 mL of CTC were applied via intraperitoneal injection to guinea pigs weighting 372 ± 3 g (low dose group) and 373 ± 6 g (high dose group); the mortility rate was determined after an observation period of 35 d, control animals were injected with distilled water
Doses:
- 0.5 and 2.0 ml (= 0.792 and 3.17 g, on average 2130 and 8500 mg/kg bw)
No. of animals per sex per dose:
20 per dose, sex not indicated
Control animals:
yes
Details on study design:
- Duration of observation period following administration: 35 days
- Frequency of weighing: daily, but not reported
- Necropsy of survivors performed: no
- Other examinations performed: clinical signs, body weight,organ weights, histopathology, other: no
Statistics:
- no statictic analysis conducted

Results and discussion

Effect levels
Sex:
not specified
Dose descriptor:
other: approximated LD50
Effect level:
> 2 130 - < 8 500 mg/kg bw
Mortality:
after 3 days: 2130 mg/kg bw 9/20
8500 mg/kg bw 10/20
after 35 days: 2130 mg/kg bw 9/20
8500 mg/kg bw 10/20
Clinical signs:
- not analyzed
Body weight:
- not repoted
Gross pathology:
- not analyzed

Applicant's summary and conclusion

Conclusions:
From the present study (Wahlberg, 1979) the LD50 (guinea pig, i.p. injection) can be approximated to lie between 2130 and 8500 mg/kg bw.
Executive summary:

The potential of the test substance CTC (named carbon tetrachloride in the study report) to induce acute toxicity after intraperitoneal injection was evaluated in a study following no official guideline. Guinea pigs, unspecified concerning sex and strain, were injected i.p. with CTC and were observed for 35 days for mortality and body weight only, but the body weight development was not reported. No pathological or histological analysis were conducted. Treatment with the the test substance resulted in mortalities of 45 % at 2130 mg/kg bw and 50 % at 8500 mg/kg bw after 35 d of observation. All deaths occured within 3 days post exposure without any further deaths during the rest of the observation period. So this effect is monophasic in contrast to the occurence of deaths after dermal application which was biphasic. As only two concentration were tested which both lead to about 50 % mortality, the LD50 could only be approximated to lie between the two tested doses. The approximated LD50 was derived to be >2000 mg/kg bw.