Registration Dossier

Administrative data

biotransformation and kinetics
Type of information:
experimental study
Adequacy of study:
key study
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Very well documented publication; no guideline available; not under GLP

Data source

Reference Type:
Uptake, distribution and effects of carbon tetrachloride in rainbow trout (Salmo gairdneri)
Statham, C.N.
Bibliographic source:
Toxicol. Appl. Pharmacol. 45, 131-140

Materials and methods

Test guideline
no guideline available
Principles of method if other than guideline:
Exposure of fish to radiolabeled CTC in water and directly by intraperitoneal injection. Evaluation of uptake, distribution and elimination measuring the 14C-CTC present in blood, bile and different tissues.
GLP compliance:
not specified
Type of medium:

Test material

Constituent 1
Chemical structure
Reference substance name:
Carbon tetrachloride
EC Number:
EC Name:
Carbon tetrachloride
Cas Number:
Molecular formula:
Details on test material:
Radioactive CTC obtained from Amersham/Searle (Illinois).

Results and discussion

Transformation products:

Applicant's summary and conclusion

Executive summary:

Effects of CTC in rainbow trout after water exposure and by intraperitoneal injection were analyzed. The fish, after a period of acclimatization (at least 1 week), were exposed to radiolabeled CTC by exposure water or directly by i.p. administration. A control group was used for both administration routes.

Fish administrated intraperitoneally were sacrificed after 6 h and blood, tissue and serum samples were assayed. The fish were also subjected to histological examination (liver, spleen). I.p. injection produced 5 -10 fold increases in serum GOT, GPT and ICD activities, without elevating liver triglyceride concentrations.Vacuolization, focal and laminar necrosis were observed in liver.

The spleen displayed severe laminar necrosis of the splenic capsule and parenchyma.

CTC administrated intraperitoneally resulted in much higher liver concentrations at all time periods, compared to water exposure groups.

Fish exposed to water with radiolabeled CTC (1 -80 mg/L ) were transferred to fresh flowing dechlorinated water after 2 hr exposure period. Approximately 22% of the radioactivity removed from the exposure water was accounted for in the fish (whole body). The remaining 78% presumably was lost to the atmosphere (static system with continuous areation). Uptake by water exposure was rapid and levels reached a plateau in liver, muscles, and blood at 15 to 30 min, but increased further in fat, suggesting

a redistribution to the adipose tissue. After 2 h of exposure, 14C-levels were highest in fat followed by liver and brain, and lowest in skin and gill. Half-lives for elimination were about 2-4 h except for live(39 h) and rates  followed first-order kinetics.

Exposure up to 80 mg/L produced neither mortality nor significant changes in enzymatic activities.