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Toxicological information

Skin irritation / corrosion

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Administrative data

Endpoint:
skin irritation: in vivo
Type of information:
experimental study
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: A scientifically sound study, but no individual data, limited methodological information.

Data source

Reference
Reference Type:
publication
Title:
Pouvoir irritant des principaux solvants chlorés aliphatiques sur la peau et les muqueuses oculaires du lapin
Author:
Duprat P, Delsaut L, Gradiski D.
Year:
1976
Bibliographic source:
Eur J Toxicol Environ Hyg., vol. 9, no. 3, p. 171-7

Materials and methods

Test guideline
Qualifier:
no guideline followed
Principles of method if other than guideline:
- application of CTC and other chlorinated solvents to the skin of rabbits
- at day 3 histologic examinations of the treated skin regions
- a non-discribed ranking system for skin lesions (differentiating between erythema and edema) was used to classify the skin lesions macroscopically
GLP compliance:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): tetrachlorure de carbone
- Analytical purity: > 99.5 %
- Lot/batch No.: not reported
- Supplier: Flucka

Test animals

Species:
rabbit
Strain:
other: New Zealand
Details on test animals and environmental conditions:
TEST ANIMALS
- Source: not reported
- Age at study initiation: not reported
- Weight at study initiation: 2.4 - 3.0 Kg
- Housing: not reported
- Diet (e.g. ad libitum): not reported
- Water (e.g. ad libitum): not reported
- Acclimation period: not reported


ENVIRONMENTAL CONDITIONS
- Temperature (°C): not reported
- Humidity (%): not reported
- Air changes (per hr): not reported
- Photoperiod (hrs dark / hrs light):not reported

Test system

Type of coverage:
not specified
Preparation of test site:
not specified
Vehicle:
not specified
Controls:
not specified
Amount / concentration applied:
TEST MATERIAL
- Amount(s) applied (volume or weight with unit): very likely 0.5 mL (methods not detailed, only referenced to other publication)
- Concentration (if solution): unclear
- Purity > 99.5 %


VEHICLE
- not reported
Duration of treatment / exposure:
24 h
Observation period:
72 h, observations at 24, 48 and 72 h post treatment
Number of animals:
6
Details on study design:
TEST SITE
- Area of exposure:
- % coverage:
- Type of wrap if used:


REMOVAL OF TEST SUBSTANCE
- Washing (if done):
- Time after start of exposure:


SCORING SYSTEM: Draize scoring system

Results and discussion

In vivo

Resultsopen allclose all
Irritation parameter:
other: I.I.C.P (very likely: primary skin irritation index)
Basis:
mean
Time point:
other: 24 and 72 h
Score:
4.2
Reversibility:
fully reversible within: 2 weeks or less
Irritation parameter:
erythema score
Time point:
24/48/72 h
Remarks on result:
not determinable
Irritation parameter:
edema score
Time point:
24/48/72 h
Remarks on result:
not determinable

Any other information on results incl. tables

- the authors classify the skin irritation potency of CTC as medium in ranking comprising the stages: non irritating, almost non irritating, medium irritant and severely irritant.

Applicant's summary and conclusion

Interpretation of results:
slightly irritating
Remarks:
Migrated information Criteria used for interpretation of results: expert judgment
Conclusions:
The present publication (Duprat 1976) states CTC to be medium irritating to the skin of rabbits after single application.
Executive summary:

Individual data from different time points of observation are not presented, so a clear classification according to OECD GHS is not possible.

The publication is a survey report on the irritative potential of different chlorinated solvents. Rabbits were dermaly exposed with CTC (and other solvents) and the skin reaction was observed macroscopically as well as microscopically and scored by an undisclosed scoring system (very likely the Draize score system). Observation periods and dosing also remains unclear.