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Toxicological information

Eye irritation

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Administrative data

Endpoint:
eye irritation: in vivo
Type of information:
experimental study
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Scientifically sound study, but no individual data, limited methodological information

Data source

Reference
Reference Type:
publication
Title:
Unnamed
Year:
1976

Materials and methods

Test guideline
Qualifier:
no guideline followed
Principles of method if other than guideline:
The system of eye irritation scoring by Kay and Calandra (John H. Kay , Joseph C. Calandra , Interpretation of eye irritation tests (1962), Journal of Cosmetic Science, Vol. 13, No. 6, pp. 281-290) was used to classify the skin lesions macroscopically
GLP compliance:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): tetrachlorure de carbone
- Analytical purity: > 99.5 %
- Lot/batch No.: not reported
- Supplier: Flucka

Test animals / tissue source

Species:
rabbit
Strain:
other: New Zealand
Details on test animals or tissues and environmental conditions:
TEST ANIMALS
- Source: not reported
- Age at study initiation: not reported
- Weight at study initiation: 2.4 - 3.0 Kg
- Housing: not reported
- Diet (e.g. ad libitum): not reported
- Water (e.g. ad libitum): not reported
- Acclimation period: not reported


ENVIRONMENTAL CONDITIONS
- Temperature (°C): not reported
- Humidity (%): not reported
- Air changes (per hr): not reported
- Photoperiod (hrs dark / hrs light):not reported

Test system

Vehicle:
not specified
Controls:
not specified
Amount / concentration applied:
TEST MATERIAL
- Amount(s) applied (volume or weight with unit): 0.1 mL
- Concentration (if solution): 100 %


VEHICLE
- not applicable
Duration of treatment / exposure:
single application, no wash out
Observation period (in vivo):
7 d
Number of animals or in vitro replicates:
6
Details on study design:
REMOVAL OF TEST SUBSTANCE
- Washing (if done): not reported



SCORING SYSTEM: Draize Scoring (see "Any other information on materials and Methods")


TOOL USED TO ASSESS SCORE: not reported

Results and discussion

In vivo

Resultsopen allclose all
Irritation parameter:
other: irritation score as defined by Kay and Calandra (1962)
Basis:
mean
Time point:
other: unclear
Score:
5
Reversibility:
fully reversible within: 2 weeks
Irritation parameter:
cornea opacity score
Time point:
24/48/72 h
Remarks on result:
not determinable
Irritation parameter:
iris score
Time point:
24/48/72 h
Remarks on result:
not determinable
Irritation parameter:
conjunctivae score
Time point:
24/48/72 h
Remarks on result:
not determinable
Irritation parameter:
chemosis score
Time point:
24/48/72 h
Remarks on result:
not determinable

Any other information on results incl. tables

- according to Kay and Calandra (1962) a score of 5 is equivalent with minimally irritating

Applicant's summary and conclusion

Interpretation of results:
slightly irritating
Remarks:
Migrated information Criteria used for interpretation of results: expert judgment
Conclusions:
The present publication (Duprat 1976) states CTC to be minimally irritating to the eye of rabbits after single application.
Executive summary:

The publication is a survey report on the irritative potential of different chlorinated solvents. Rabbits were ocular exposed with CTC (and other solvents) and the reaction of the eye was observed macroscopically as well as microscopically and scored by the scoring system of Kay and Calandra (1962). Individual data from different time points of observation are not presented. The effects are reported to be reversible within 1 to 2 weeks.