Quinoline

Acute Toxicity: dermal

Administrative data

Endpoint:

acute toxicity: dermal

Type of information:

experimental study

Adequacy of study:

key study

Study period:

Jun-Jul 1981

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Study not performed according to GLP but it is similar to the OECD 402 guideline however the substance tested is poorly described.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1981

Report date:

1981

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 402 (Acute Dermal Toxicity)

GLP compliance:

no

Test type:

standard acute method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

Quinoline

EC Number:

202-051-6

EC Name:

Quinoline

Cas Number:

91-22-5

Molecular formula:

C9H7N

IUPAC Name:

quinoline

Details on test material:

- Name of test material (as cited in study report): 110.831
- Substance type: no data
- Physical state: yellowish liquid
- Analytical purity: no data
- Impurities (identity and concentrations): no data
- Composition of test material, percentage of components: no data
- Isomers composition: no data
- Purity test date: no data
- Lot/batch No.: no data
- Expiration date of the lot/batch: not determined
- Stability under test conditions: no data
- Storage condition of test material: at room temperature, day light

Test animals

Species:

rat

Strain:

Sprague-Dawley

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: Dr A Ivanovas, Kissleg/Allgäu
- Age at study initiation: no data
- Weight at study initiation: male: 220-270 g, female: 200-245 g
- Fasting period before study: no data
- Housing: individually
- Diet: Ssniff R10 (pellets) ad libitum
- Water (e.g. ad libitum): yes
- Acclimation period: no data


ENVIRONMENTAL CONDITIONS
- Temperature (°C): 22 +/- 1
- Humidity (%): no data
- Air changes (per hr): no data
- Photoperiod (hrs dark / hrs light): 12/12


IN-LIFE DATES: From: June To: July 1981

Administration / exposure

Type of coverage:

occlusive

Vehicle:

polyethylene glycol

Details on dermal exposure:

TEST SITE
- Area of exposure: back, area 5 x 7.5 cm
- % coverage: no data
- Type of wrap if used: aluminium foil and "Elastoplast"


REMOVAL OF TEST SUBSTANCE
- Washing (if done): yes with lukewarm water
- Time after start of exposure: 24 h


TEST MATERIAL
- Amount(s) applied (volume or weight with unit): 3 mL / kg
- Concentration (if solution): no data
- Constant volume: yes


VEHICLE
- Amount(s) applied (volume or weight with unit): no data
- Concentration (if solution): no data
- Lot/batch no. (if required): no data
- Purity: no data

Duration of exposure:

24 h

Doses:

0.82 - 1.0 - 1.21 - 1.47 mL/kg bw

No. of animals per sex per dose:

5

Control animals:

yes, concurrent vehicle

Details on study design:

- Duration of observation period following administration: 14 days
- Frequency of observations and weighing: once a day for observations, at day 1, 7 and 15 for weighing
- Necropsy of survivors performed: yes

Statistics:

Finney method

Results and discussion

Preliminary study:

not relevant

Effect levels

Sex:

male/female

Dose descriptor:

LD50

Effect level:

1.26 mL/kg bw

95% CL:

1.15 - 1.39

Mortality:

See table 1

Clinical signs:

Skin effect:
Neither erythem nor oedema was observed.
Clinical signs:
Decrease of motility, abdominal posture of pre-agony.

Body weight:

No effect on body weight gain.

Gross pathology:

No effects in the animal that died in the study.
Animals sacrified at the end of the study:
Dose 1 mL/kg: Pale discoloration of liver and kidneys, bloody aspect of the stomach and intestine content (1 female)
Dose 1.21 mL/kg: Autolysis (1 male), Pale discoloration of liver and kidneys, medium grade hyperemia of the lungs. Bloody aspect of the stomach and small intestine content (2 females)
Dose 1.47 mL/kg: Autolysis (2 males and 5 females), Pale discoloration of liver and kidneys (2 males), medium grade hyperemia of the lungs (2 males). Bloody aspect of the stomach (2 males and 3 females) and small intestine content (2 males).

Other findings:

none

Any other information on results incl. tables

Table 1: Mortality

Time	Control		0.82 mL/kg	1.0 mL/kg	1.21 mL/kg	1.47 mL/kg
Up to 6h	M F	0/5 0/5	0/5 0/5	0/5 0/5	0/5 0/5	0/5 0/5
6-24h	M F	0/5 0/5	0/5 0/5	0/5 0/5	0/5 0/5	0/5 0/5
24-48h	M F	0/5 0/5	0/5 0/5	0/5 1/5	0/5 1/5	4/5 4/5
3-7 d	M F	0/5 0/5	0/5 0/5	0/5 0/5	1/5 1/5	0/5 1/5
7-14d	M F	0/5 0/5	0/5 0/5	0/5 0/5	0/5 0/5	0/5 0/5
Total	M F	0/5 0/5	0/5 0/5	0/5 1/5	1/5 2/5	4/5 5/5
%		0	0	10	30	90

Applicant's summary and conclusion

Interpretation of results:

harmful

Remarks:

Migrated information Criteria used for interpretation of results: EU

Conclusions:

The dermal LD50 of quinoline in rat is 1377 mg/kg. Quinoline is harmful in contact with skin according to the 67/548/EEC directive and according to CLP (cat 4).

Executive summary:

A standard dermal acute toxicity method has been performed with quinoline on rat. 5 animals per sex per group were treated by quinoline doses from 0.82 to 1.47 mL/kg bw. In these conditions, the LD50 is 1.26 mL/kg corresponding to 1377 mg/kg bw.