Registration Dossier

Administrative data

Endpoint:
sediment toxicity: short-term
Type of information:
experimental study
Adequacy of study:
disregarded due to major methodological deficiencies
Study period:
no data
Reliability:
3 (not reliable)
Rationale for reliability incl. deficiencies:
other: The test conditions are not standardized and the mortality in the control is quite high. No detail is available about the test substance and the results.

Data source

Reference
Reference Type:
publication
Title:
A Chironomus tentans Bioassay for Testing Synthetic Fuel Products and Effluents, with Data on Acridine and Quinoline
Author:
Cushman RM, McKamey MI
Year:
1981
Bibliographic source:
Bull Contam Toxicol 26: 601-605

Materials and methods

Test guideline
Qualifier:
no guideline followed
Principles of method if other than guideline:
Exposure of chironomes to quinoline dissolved in water, no sediment included in the study but artificial substrat.
GLP compliance:
not specified

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
no data

Sampling and analysis

Analytical monitoring:
yes

Test substrate

Vehicle:
no
Details on sediment and application:
Glass beakers (100-mL capacity) were filled with 80 mL of filtered (0.45 m) well water. To each beaker approximately 0.3 g of glass wool was added as a substrate. All beakers were covered with cardboard boxes to exclude light throughout the tests; no aeration was provided.

Test organisms

Test organisms (species):
Chironomus dilutus (previous name: Chironomus tentans)
Details on test organisms:
Chironomus tentans egg masses were obtained from Battelle, Pacific Northwest Laboratory, Richland, Washington, and aquarium-reared. Each 35-liter aquarium was filled to a depth of approximately 8 cm with well water [pH 7.8, alkalinity 120 mg/L, hardness 140 mg/L]. A substrate of shredded paper towel was provided, and each aquarium was aerated. Cultures were fed weekly a suspension of ground dry dog food in water. Water temperature in the aquaria was 20 to 25 °C. Aquaria received about 12 h of light daily.

Four-instar larvae were used in the test.

Study design

Study type:
laboratory study
Test type:
static
Water media type:
freshwater
Type of sediment:
artificial sediment
Limit test:
no
Exposure duration
Duration:
48 h
Exposure phase:
total exposure duration
Post exposure observation period:
no

Test conditions

Hardness:
140 mg/L
Test temperature:
23 to 26°C
pH:
7.8
Dissolved oxygen:
no data
Salinity:
not relevant
Ammonia:
no data
Nominal and measured concentrations:
no detail
Reference substance (positive control):
no

Results and discussion

Effect concentrations
Duration:
48 h
Dose descriptor:
LC50
Effect conc.:
57.2 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: 54.8-59.2 mg/L
Details on results:
no further data
Results with reference substance (positive control):
not performed

Any other information on results incl. tables

The mortality in control group was 11.5%, the pupation rate in the control was 6.1%.

Applicant's summary and conclusion

Validity criteria fulfilled:
no
Remarks:
control mortality exceeds the 10% value
Conclusions:
Even if no OECD guideline is available for chironomus acute toxicity, the 10% mortality of the control comes from US EPA guideline on macroinvertebrates.
Executive summary:

An acute toxicity test has been performed with Chironomus tentans fourth instar larvae. They were exposed for 48 h to different concentrations of quinoline and mortality was recorded. The sediment was artificial: glass wool.

Water concentration of quinoline was measured at the beginning and at the end of the study.

In these conditions the LC50 (48h) is 57.2 mg/L, the control mortality was 11.5%.