Registration Dossier

Toxicological information

Specific investigations: other studies

Currently viewing:

Administrative data

Endpoint:
biochemical or cellular interactions
Type of information:
experimental study
Adequacy of study:
supporting study
Study period:
No data
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Well described study.

Data source

Reference
Reference Type:
publication
Title:
Activation of the Human Ah Receptor by Aza-Polycyclic Aromatic Hydrocarbons and Their Halogenated Derivatives
Author:
Saeki K, Matsuda T, Kato T, Yamada K, Mizutani T, Matsui S, Fukuhara K, Miyata N
Year:
2003
Bibliographic source:
Biol Pharm Bull 26(4):448-452

Materials and methods

Test guideline
Qualifier:
no guideline available
Principles of method if other than guideline:
AhR ligand activity was measured by using the yeast AhR signaling assay. The genes of the human AhR and its heterodimer partner, AhR nuclear translocator (Arnt), are co-expressed, and AhR ligand activity can be detected and quantified by measuring the beta-galactosidase activity resulting by AhR-mediated transcriptional activation of a lacZ reporter plasmid.
GLP compliance:
not specified
Type of method:
in vitro
Endpoint addressed:
not applicable

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
Supplier: Aldrich
No further data

Administration / exposure

Route of administration:
other: incubation
Vehicle:
not specified
Analytical verification of doses or concentrations:
no
Duration of treatment / exposure:
see below
Frequency of treatment:
once
Post exposure period:
none
Doses / concentrations
Remarks:
Doses / Concentrations:
50 to 500 µM
Basis:

No. of animals per sex per dose:
not relevant

Examinations

Examinations:
not relevant
Positive control:
benzo[a]pyrene

Results and discussion

Details on results:
Concentration producing lacZ units equal to 50% of the max response of the positive substance: EC(BAP50) > 500 µM for quinoline.

Applicant's summary and conclusion

Conclusions:
Quinoline is about three orders of magnitude less active than BaP.
Executive summary:

Aryl hydrocarbon receptor (AhR) is a ligand-activated transcription factor through which dioxins and carcinogenic polycyclic aromatic hydrocarbons cause altered gene expression and toxicity.

A screening assay has been performed using the yeast AhR signaling assay.

Quinoline does not activate this receptor in a significant extent.