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Registration Dossier
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EC number: 204-873-0 | CAS number: 127-95-7
Toxicity to terrestrial plants
Administrative data
- Endpoint:
- toxicity to terrestrial plants: long-term
- Type of information:
- migrated information: read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Study period:
- No data
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: No guideline study used
Data source
Referenceopen allclose all
- Reference Type:
- publication
- Title:
- PH restraints on lettuce fruit germination
- Author:
- Reynolds T
- Year:
- 1 975
- Bibliographic source:
- Ann. Bot. 39: 797-805
- Reference Type:
- publication
- Title:
- Characterization of osmotic restraints on lettuce fruit germination
- Author:
- Reynolds T
- Year:
- 1 975
- Bibliographic source:
- Ann. Bot. 39: 791-796
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: Reynolds T. (1975) Characterization of osmotic restraints on lettuce fruit germination
- Deviations:
- not applicable
- Principles of method if other than guideline:
- Examination of the effects of buffers with a range of pH values and of concentrations low enough to exert negligible osmotic stress on germination of lettuce fruits.
- GLP compliance:
- no
Test material
- Reference substance name:
- Oxalic acid
- EC Number:
- 205-634-3
- EC Name:
- Oxalic acid
- Cas Number:
- 144-62-7
- IUPAC Name:
- oxalic acid
- Details on test material:
- No data
Constituent 1
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material (migrated information):
No data
Sampling and analysis
- Analytical monitoring:
- no
- Details on sampling:
- No data
Test substrate
- Vehicle:
- no
- Details on preparation and application of test substrate:
- No data
Test organisms
- Species:
- Lactuca sativa
- Plant group:
- Dicotyledonae (dicots)
- Details on test organisms:
- No data
Study design
- Test type:
- seed germination/root elongation toxicity test
- Study type:
- laboratory study
- Substrate type:
- other: agar
- Limit test:
- no
- Total exposure duration:
- 72 h
- Post exposure observation period:
- No data
Test conditions
- Test temperature:
- 29±0.5°C
- pH:
- 2.25
- Moisture:
- No data
- Details on test conditions:
- TEST SYSTEM
- Testing facility: laboratory
- Test container (type, material, size): aluminium boxes (4.2x2.1x1.6 cm) with glass windows in the lids
- Amount of soil: 0.5 percent agar (8 ml) in aluminium boxes in which appropriate weights of the test substances were dissolved.
0.5 per cent agar (10 ml) in polystyrene pots in which appropriate weights of the test substances were dissolved
- Method of seeding:
- No. of seeds per container: Fifty fruits of Lactuca sativa L. cv. Arctic King (Sutton and Son Ltd) were sown in aluminium boxes
Twenty lettuce fruits were sown in polystyrene pots (2.8 x 3.5 cm) closed by lids floating in the water baths.
Each set contained five replicate pots
GROWTH CONDITIONS
- Light intensity and quality: light conditions according to Reynolds and Thompson (1973)
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Germination at single temperatures was determined by means of thermostatically controlled water baths (±0.5°C).
germination in the pots was counted after 72 h (Reynolds, 1973). Osmotic potentials are given as calculated and have not been determined physicallyAny discrepanties between theoretical and actual values have been ignored for the purposes of this investigation. - Nominal and measured concentrations:
- calculated
- Reference substance (positive control):
- not specified
Results and discussion
Effect concentrations
- Species:
- Lactuca sativa
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 8 other: mM
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- germination
- Remarks on result:
- other: 1.4
- Details on results:
- No data
- Results with reference substance (positive control):
- No data
- Reported statistics and error estimates:
- No data
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The 72 h EC50 was 8 mM.
- Executive summary:
Lettuce (Lactuca sativa) was exposed to oxalic acid during 72 h. No guideline was used. A 72 h EC50 of 8 mM was determined.
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