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Toxicological information

Repeated dose toxicity: oral

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Administrative data

Endpoint:
sub-chronic toxicity: oral
Type of information:
experimental study
Adequacy of study:
supporting study
Study period:
no data
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Altough no specific guideline was followed, the study is well performed and reported. Main focus was on growth and reproduction.

Data source

Reference
Reference Type:
publication
Title:
Effect of dietary ingestion of oxalic acid on growth and reproduction in male and female long-evans rats
Author:
Goldman, M., Doering, G.J., Nelson, R.G.
Year:
1977
Bibliographic source:
Research Communications in Chemical Pathology and Pharmacology, Vol 18, No 2, p 369-372

Materials and methods

Test guideline
Qualifier:
no guideline followed
Principles of method if other than guideline:
Male and female rats received a diet of Purina laboratory chow supplemented with 2.5 and 5.0% oxallic acid for a period of 70 days. Food and water intake was observed daily, and body weight at appropriate intervals. Furthermore, vaginal smears were examined daily, and at necropsy a number of visceral and endocrine tissues were weighed.
GLP compliance:
not specified
Limit test:
no

Test material

Constituent 1
Reference substance name:
Oxalic acid
EC Number:
205-634-3
EC Name:
Oxalic acid
Cas Number:
144-62-7
IUPAC Name:
oxalic acid

Test animals

Species:
rat
Strain:
Long-Evans
Sex:
male/female

Administration / exposure

Route of administration:
oral: feed
Vehicle:
not specified
Analytical verification of doses or concentrations:
not specified
Duration of treatment / exposure:
70 days
Frequency of treatment:
via food ad libitum
Doses / concentrationsopen allclose all
Remarks:
Doses / Concentrations:
2.5%
Basis:
nominal in diet
Remarks:
Doses / Concentrations:
5.0%
Basis:
nominal in diet
No. of animals per sex per dose:
Controls: 12 males, 12 females
2.5%: 11 males, 11 females
5.0%: 9 males, 10 females
Control animals:
yes, concurrent no treatment

Results and discussion

Effect levels

open allclose all
Dose descriptor:
dose level: 2.5%
Sex:
male/female
Basis for effect level:
other: Mortality rate was less than 10%, no external untoward effects were observed. In the vaginal smears, some diestrus and persistent vaginal cornification was observed. Histological examinations of ovarian and tested tissue showed abnormalities.
Remarks on result:
not measured/tested
Remarks:
Effect level not specified (migrated information)
Dose descriptor:
dose level: 5%
Basis for effect level:
other: Mortality rate was 25%, animals appeared emaciated, stunted, and gaunt with arched backs. Furthermore, growth rates, body weight, visceral, endocrine and accessory reproductive tissue weight were decreased.
Remarks on result:
not measured/tested
Remarks:
Effect level not specified (migrated information)

Target system / organ toxicity

Critical effects observed:
not specified

Applicant's summary and conclusion

Conclusions:
Dietary ingestion of 2.5% or 5% oxalic acid over a 2-month interval resulted in marked depression in growth rate, body weight, visceral, endocrine, and accessory reproductive tissue weights, particularly in the highest concentration, under the current test conditions.
Executive summary:

Male and female rats received a diet of Purina laboratory chow supplemented with 2.5 and 5.0% oxallic acid for a period of 70 days. Dietary ingestion of 2.5% or 5% oxalic acid over a 2-month interval resulted in marked depression in growth rate, body weight, visceral, ensocrine, and accessory reproductive tissue weights, particularly in the highest concentration, under the current test conditions.

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