Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 225-443-9 | CAS number: 4851-50-7
A study with activated sludge was performed according to the German standard method "Amtsblatt der EG L 133 Teil C: Biologische Abbaubarkeit: Prüfung der Atmungshemmung", which is equivalent to the OECD Guideline 209. Two controls without test item were included in the test design, one at the start and the other at the end of the test series. Each batch of activated sludge was checked using 3,5-Dichlorophenol as a reference compound. The physico-chemical oxygen consumption control should be carried out, since some substances may consume oxygen by chemical reactivity. In order to be able to differentiate between physico-chemical oxygen consumption and biological oxygen consumption (respiration), at least the maximum concentration of the test item was additionally tested without activated sludge. To measure the oxygen consumption, 250 mL of sludge with test item (or control or reference compound) was incubated for 3 h in 300 mL closed Erlenmeyer flasks (with air inlet and outlet) and aerated through a glass tube at 50-100 L/h with clean oil-free air. For measurement, the content of the Erlenmeyer flasks was completely transferred to 250 mL BOD bottles and O2-content was measured with an O2-meter (redox electrode) with writer. The respiration rate for each concentration was determined graphically from the linear part of the curve of O2-content versus time. The inhibitory effect of the test item at a particular concentration is expressed as a percentage of the mean of the respiration rates of two controls. The sludge was exposed to three different nominal concentrations (100, 1000, 10000 mg/L) of Solvent Green 28. Because of the strong oxygen consumption of the activated sludge, only 0.48 g/L was used in all batches. The respiration rate of each mixture was determined after aeration period of 30 min. Solvent Green 28 showed 7.4 % respiration inhibition of activated sludge at the highest test item concentration of 10000 mg/L. An EC50 >= 10000 mg/L was determined. Test concentrations are given as nominal concentrations and were not confirmed by analytical methods. This toxicity study is classified as acceptable and satisfies the guideline requirements for the toxicity study to microorganisms.
In a grouping approach data from Solvent violet 36 are used for Solvent green 28. The study showed 7.4 % respiration inhibition of activated sludge at the highest test item concentration (10000 mg/L) and an EC50 of higher than 10000 mg/L was determined. Test concentrations are given as nominal concentrations.
"Should read: EC50 > 10000 mg/L"
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
Welcome to the ECHA website. This site is not fully supported in Internet Explorer 7 (and earlier versions). Please upgrade your Internet Explorer to a newer version.
Close Do not show this message again