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Short-term toxicity to aquatic invertebrates

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Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2001
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study without detailed documentation
Qualifier:
according to guideline
Guideline:
EU Method C.2 (Acute Toxicity for Daphnia)
Deviations:
no
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Deviations:
not applicable
GLP compliance:
yes (incl. QA statement)
Analytical monitoring:
no
Vehicle:
no
Details on test solutions:
Pre-treatment of test item and preparation of test item concentrations:
- The test item was added to test medium at a nominal co.ncentration of 1 00 mg/L
- The test substance tended to float on the water surface and therefore the mixture was shaken vigorously with a vortex apparatus and treated with ultrasonic waves for 5 minutes
- Afterwards magnetic stirring was applied for a total period of three days in order to achieve maximum· saturation of the test medium with the test
substance
- After stirring the 100 mg/L solution appeared to be a greyish green turbid dispersion with a floating layer
- One part of this 100 mg/L solution was filtered (Schleicher & Schuell 604 filter) in order to remove the larger (i.e. >circa 5 µm) undissolved test
substance particles
- The filtered test solution was clear and colourless
- The other part of the 100 mg/L was not filtered and used as such
Test organisms (species):
Daphnia magna
Details on test organisms:
- Characteristics: For the test selection of young daphnia with an age of < 24 hours
Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
48 h
Hardness:
218 mg/L CaCO3
Test temperature:
20.1-20.7 °C
pH:
7.9-8.0
Dissolved oxygen:
8.2-8.6 mg/l
Salinity:
n.a.
Conductivity:
no data
Nominal and measured concentrations:
100 mg/L test item
Details on test conditions:
EXPOSURE CONDITIONS:
- Test concentrations: filtered solution prepared at a nominal concentration of 100 mg/L; unfiltered solution prepared at a nominal concentration
of 100 mg/L; Blank-control medium
- Loading: 10 Daphnia per vessel containing 80 ml test solution
- Number of replicates: 2 replicates for each concentration
Reference substance (positive control):
no
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Key result
Duration:
48 h
Dose descriptor:
NOEC
Effect conc.:
100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Validity criteria fulfilled:
yes
Remarks:
(Immobilisation and other abnormalities in the controls did not exceed 10% by the end of the test; dissolved oxygen concentration remained above 3 mg/L throughout the exposure period)
Conclusions:
Under the conditions of the present study Solvent green 28 did not induce acute immobilisation of Daphnia magna at a nominal concentration of 100 mg/L after 48 hours of exposure (NOEC).
Executive summary:

Acute toxicity to Daphnia magna was determined in accordance with the OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test). In order to test acute toxicity to invertebrates of the substance, Under the conditions of the present study Solvent green 28 did not induce acute immobilisation of Oaphnia magna at a nominal concentration of 100 mg/L after 48 hours of exposure (NOEC).

This toxicity study is classified as acceptable and satisfies the guideline requirements for the acute Daphnia study.

Description of key information

Under the conditions of the present study Solvent green 28 did not induce acute immobilisation of Daphnia magna at a nominal concentration of 100 mg/L after 48 hours of exposure (NOEC).

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates
Effect concentration:
100 mg/L

Additional information

"Should read: EC 50 >100 mg/L"