Registration Dossier
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EC number: 225-443-9 | CAS number: 4851-50-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2001
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study without detailed documentation
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Deviations:
- no
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- not applicable
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- Pre-treatment of test item and preparation of test item concentrations:
- The test item was added to test medium at a nominal co.ncentration of 1 00 mg/L
- The test substance tended to float on the water surface and therefore the mixture was shaken vigorously with a vortex apparatus and treated with ultrasonic waves for 5 minutes
- Afterwards magnetic stirring was applied for a total period of three days in order to achieve maximum· saturation of the test medium with the test
substance
- After stirring the 100 mg/L solution appeared to be a greyish green turbid dispersion with a floating layer
- One part of this 100 mg/L solution was filtered (Schleicher & Schuell 604 filter) in order to remove the larger (i.e. >circa 5 µm) undissolved test
substance particles
- The filtered test solution was clear and colourless
- The other part of the 100 mg/L was not filtered and used as such - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- - Characteristics: For the test selection of young daphnia with an age of < 24 hours
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 48 h
- Hardness:
- 218 mg/L CaCO3
- Test temperature:
- 20.1-20.7 °C
- pH:
- 7.9-8.0
- Dissolved oxygen:
- 8.2-8.6 mg/l
- Salinity:
- n.a.
- Conductivity:
- no data
- Nominal and measured concentrations:
- 100 mg/L test item
- Details on test conditions:
- EXPOSURE CONDITIONS:
- Test concentrations: filtered solution prepared at a nominal concentration of 100 mg/L; unfiltered solution prepared at a nominal concentration
of 100 mg/L; Blank-control medium
- Loading: 10 Daphnia per vessel containing 80 ml test solution
- Number of replicates: 2 replicates for each concentration - Reference substance (positive control):
- no
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Key result
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Validity criteria fulfilled:
- yes
- Remarks:
- (Immobilisation and other abnormalities in the controls did not exceed 10% by the end of the test; dissolved oxygen concentration remained above 3 mg/L throughout the exposure period)
- Conclusions:
- Under the conditions of the present study Solvent green 28 did not induce acute immobilisation of Daphnia magna at a nominal concentration of 100 mg/L after 48 hours of exposure (NOEC).
- Executive summary:
Acute toxicity to Daphnia magna was determined in accordance with the OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test). In order to test acute toxicity to invertebrates of the substance, Under the conditions of the present study Solvent green 28 did not induce acute immobilisation of Oaphnia magna at a nominal concentration of 100 mg/L after 48 hours of exposure (NOEC).
This toxicity study is classified as acceptable and satisfies the guideline requirements for the acute Daphnia study.
Reference
Description of key information
Under the conditions of the present study Solvent green 28 did not induce acute immobilisation of Daphnia magna at a nominal concentration of 100 mg/L after 48 hours of exposure (NOEC).
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 100 mg/L
Additional information
"Should read: EC 50 >100 mg/L"
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