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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Toxicological information

Eye irritation

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Administrative data

Endpoint:
eye irritation: in vivo
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1989
Report date:
1989

Materials and methods

Principles of method if other than guideline:
Macrolexgrün G was applied to the eyes of 2 rabbits. Post observation time was 7 days.
GLP compliance:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
1,4-bis[[4-(1,1-dimethylethyl)phenyl]amino]-5,8-dihydroxyanthraquinone
EC Number:
225-443-9
EC Name:
1,4-bis[[4-(1,1-dimethylethyl)phenyl]amino]-5,8-dihydroxyanthraquinone
Cas Number:
4851-50-7
Molecular formula:
C34H34N2O4
IUPAC Name:
1,4-bis[(4-tert-butylphenyl)amino]-5,8-dihydroxy-9,10-dihydroanthracene-9,10-dione

Test animals / tissue source

Species:
rabbit
Strain:
not specified

Test system

Vehicle:
not specified
Controls:
not required
Amount / concentration applied:
ca. 50 mg
Duration of treatment / exposure:
7 days (eyes were not washed).
Observation period (in vivo):
7 days
Number of animals or in vitro replicates:
2 animals

Results and discussion

In vivo

Resultsopen allclose all
Irritation parameter:
cornea opacity score
Basis:
mean
Time point:
24/48/72 h
Score:
0
Max. score:
4
Reversibility:
other: score = 0 at any time point
Irritation parameter:
iris score
Basis:
mean
Time point:
24/48/72 h
Score:
0
Max. score:
2
Reversibility:
other: score = 0 at any time point
Irritation parameter:
conjunctivae score
Basis:
mean
Time point:
24/48/72 h
Score:
0.2
Max. score:
3
Reversibility:
fully reversible within: 48 hours
Irritation parameter:
chemosis score
Basis:
mean
Time point:
24/48/72 h
Score:
0
Max. score:
4
Reversibility:
other: score = 0 at any time point
Irritant / corrosive response data:
Cornea opacity, iris and chemosis score were 0 (24,48, 72 hours). The conjunctivae score was 0.2 (24, 48, 72 hours). It was fully reversible within 48 hours.

Applicant's summary and conclusion

Interpretation of results:
GHS criteria not met
Conclusions:
Not irritating. Cornea opacity, iris and chemosis score were 0 (24,48, 72 hours). The conjunctivae score was 0.2 (24, 48, 72 hours). It was fully reversible within 48 hours.
Executive summary:

Macrolexgrün G was applied to the eyes of 2 rabbits. Post observation time was 7 days. Cornea opacity, iris and chemosis score were 0 (24,48, 72 hours). The conjunctivae score was 0.2 (24, 48, 72 hours). It was fully reversible within 48 hours. Macrolexgrün G was not irritating in this test.