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Toxicological information

Genetic toxicity: in vivo

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Administrative data

Endpoint:
in vivo mammalian cell study: DNA damage and/or repair
Remarks:
Type of genotoxicity: DNA damage and/or repair
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study

Data source

Reference
Reference Type:
publication
Title:
Unnamed
Year:
1985

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to guideline
Guideline:
OECD Guideline 486 (Unscheduled DNA Synthesis (UDS) Test with Mammalian Liver Cells in vivo)
Qualifier:
according to guideline
Guideline:
EU Method B.39 (Unscheduled DNA Synthesis (UDS) Test with Mammalian Liver Cells In Vivo)
GLP compliance:
yes
Type of assay:
unscheduled DNA synthesis

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
TNT, > 99% purity

Test animals

Species:
rat
Strain:
other: Alderley Park (AP) or Fischer 344 (F344)
Sex:
male

Administration / exposure

Route of administration:
oral: gavage
Duration of treatment / exposure:
12 hours
Frequency of treatment:
adminitered once
Doses / concentrations
Remarks:
Doses / Concentrations:
100, 200, 500, 1000 mg/kg
Basis:
actual ingested
No. of animals per sex per dose:
2, 3 or 5 animals per dose
Control animals:
yes

Examinations

Tissues and cell types examined:
Liver, blood.

Results and discussion

Test results
Key result
Sex:
male
Genotoxicity:
negative
Toxicity:
no effects

Any other information on results incl. tables

TNT was inactive in each strain. Urine of the TNT-treated animals esd bright red due to the presence of TNT (in an acid medium) and their blood contained elevated levels of methaemoglobin. Nonetheless, hepatocyte, morphology was normal and showed no evidence of toxic picnosis.

Applicant's summary and conclusion

Conclusions:
Interpretation of results (migrated information): negative
Negative response observed for TNT in the liver assay indicates that it is unlikely to be rat hepatocarcinogen. Nonetheless, high levels, of methaemoglobin were observed in the TNT-trerated rats and their urine was coloured red. These facts, together with the known toxicities of this agent suggest a possible carcinogenic hazard to the haemopoetic and urinary tissue of animals exposed chronically to it at toxic dose-levels.