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Toxicological information

Acute Toxicity: oral

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Administrative data

Endpoint:
acute toxicity: oral
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
comparable to guideline study

Data source

Reference
Reference Type:
publication
Title:
Unnamed
Year:
1982

Materials and methods

Test guideline
Qualifier:
equivalent or similar to
Guideline:
OECD Guideline 401 (Acute Oral Toxicity)
GLP compliance:
no
Remarks:
No information on use of OECD and EU guidelines nor GLP conditions. The study was probably conducted according to TSCA guidelines
Test type:
standard acute method

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
alpha-TNT was obtained from E.I. du Pont de Nemours. The compound was >99% pure based on chemical and chromatographic analyses.

Test animals

Species:
rat
Strain:
Sprague-Dawley
Sex:
male/female

Administration / exposure

Route of administration:
oral: feed
Vehicle:
corn oil
Doses:
1 ml per 100 g of body weight
No. of animals per sex per dose:
4 dose levels were used, and 10 males and 10 females received each dose

Results and discussion

Effect levelsopen allclose all
Sex:
male
Dose descriptor:
LD50
Effect level:
ca. 1 320 mg/kg bw
95% CL:
95
Sex:
female
Dose descriptor:
LD50
Effect level:
ca. 795 mg/kg bw
95% CL:
95

Any other information on results incl. tables

The LD50s and confidence intervals were calculated by the method of Thomson and Weil (Weil, 1952).

The effects of short-term exposure to alpha-TNT (up to 4 weeks) appear to be almost totally reversible. At the end of a 4 week period, the body weights of rats at the high dose level were still slightly lower (not significantly) than those of controls, and there were lingerinig signs of overcompensation to the anemia in females receiving 0.25% alpha-TNT. Thus at 0.05% alpha-TNT, liver-to-blood weight ratio in females remained altered (in an apparently dose-related manner) at the end of the recovery period. At 0.25% alpha-TNT, the digns of residual toxicity were clearer. At the end od the 4 week cerovery period, the body weights of females remained significantly depressed, they still had signs of anemia, and their spleens and livers remained enlarged. After the recovery period, the testes and kidney weights of males were lower than those of controls, and there was slightl granulocytosis in the males. these factors-the cumulative nature of alpha-TNT toxicity and more long-lasting effects after longer exposure periods for some parameters - are design considerations for longer term studies to assess hazards to humans.

Applicant's summary and conclusion

Interpretation of results:
Category 4 based on GHS criteria
Conclusions:
Harmful if swallowed. According to Regulation (EC) No. 1272/2008 (EU CLP).