Registration Dossier

Administrative data

Endpoint:
skin irritation / corrosion, other
Remarks:
The study is in vivo as the study already available based on literature already published.
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1975=1978
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Due to absence of information on number of animal reliability category according to Klimisch is 3 (red score). Evaluator proposal is 2 (“reliable with restrictions”) because number of animals in compliance with method can be various.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1975
Report Date:
1975

Materials and methods

Test guideline
Qualifier:
equivalent or similar to
Guideline:
OECD Guideline 404 (Acute Dermal Irritation / Corrosion)
GLP compliance:
no
Remarks:
The study was conducted in 1975 whereas OECD and EU Guidelines as well as GLP Principles were implemented in 1981 and 1988

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
TNT was purchased from K&K Laboratories (Cleveland, Ohio). Purity >99%.

Test animals

Species:
rabbit
Strain:
other: New Zealand

Test system

Type of coverage:
other: intact and abraded skin
Preparation of test site:
shaved
Vehicle:
other: 50% paste with peanut oil prepared just prior to application
Controls:
other: 67% suspension of lactose in peanut oil was used
Amount / concentration applied:
50% paste with peanut oil
Duration of treatment / exposure:
24 and 72h
Observation period:
24 and 72h
Number of animals:
6 rabbbits with intact and abraded skin in each test groups

Results and discussion

In vivo

Results
Remarks on result:
probability of mild irritation
Other effects:
A red pigment appeared on the skin after the TNT was applied (red color under all patches at 24h). Mild irritant.

Any other information on results incl. tables

Slightly irritating.

Applicant's summary and conclusion

Interpretation of results:
GHS criteria not met
Conclusions:
Not irritating according to Regulation (EC) no 1272/2008 (CLP).