Registration Dossier

Administrative data

Endpoint:
eye irritation, other
Remarks:
The study is in vivo as the study already available based on literature already published.
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1975-1978
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Due to absence of information on number of animal reliability category according to Klimisch is 3 (red score). Evaluator proposal is 2 (“reliable with restrictions”) because number of animals in compliance with method can be various.

Data source

Reference
Reference Type:
other company data
Title:
Unnamed
Year:
1975
Report Date:
1975

Materials and methods

Test guideline
Qualifier:
equivalent or similar to
Guideline:
OECD Guideline 405 (Acute Eye Irritation / Corrosion)
GLP compliance:
no
Remarks:
The study was conducted in 1975 whereas OECD and EU Guidelines as well as GLP Principles were implemented in 1981 and 1988

Test material

Reference
Name:
Unnamed
Type:
Constituent
Type:
Constituent
Details on test material:
TNT was purchased from K&K Laboratories (Cleveland, Ohio). Purity >99%.

Test animals / tissue source

Species:
rabbit
Strain:
other: New Zealand

Test system

Vehicle:
other: 50% paste with peanut oil prepared just prior to application
Controls:
other: 67% suspension of lactose in peanut oil was used
Amount / concentration applied:
50% paste with peanut oil
Duration of treatment / exposure:
24 and 72h.
Observation period (in vivo):
24 and 72h.
Number of animals or in vitro replicates:
6 rabbits per test groups.

Results and discussion

In vivo

Results
Remarks on result:
no indication of irritation
Other effects:
Red pigment appeared around the eye after TNT was applied (red color around the eye at 24h). TNT did not produce eye irritation.

Any other information on results incl. tables

Not irritating.

Applicant's summary and conclusion

Interpretation of results:
not irritating
Conclusions:
Not irritating according to Regulation (EC) no 1272/2008 (CLP).