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EC number: 203-794-9
CAS number: 110-71-4
Test item concentration % (w/v)
number of lymph nodes
DPM per lymph nodeb)
BG = Background (1 ml 5% trichloroacetic acid) in duplicate 1 =
Control Group 2-4 = Test Group S.I. = Stimulation Index
a) = The mean value was taken from the figures BG I and
BG II b) = Since the lymph nodes of the animals of a dose
group were pooled, DPM/node was determined by dividing the measured
value by the number of lymph nodes pooled
The EC3 value could not be calculated, since all S.I.´s are below
deaths occurred during the study period.
Clinical Signs: No
symptoms of local toxicity at the ears of the animals and no systemic
findings were observed during the study period.
Body Weights: The
body weight of the animals, recorded prior
to the first application and prior to treatment with3HTdR,
was within the range commonly recorded for animals of this strain and
Ethylene glycol dimethyl ether and Diethylene glycol methyl ethyl ether,
which is tested for its sensitising potential, belong to the glycol
ether family. These substances have been demonstrated to be very similar
in structure, physical/chemical properties and the toxicological
profile. Due to the fact that Ethylene glycol dimethyl ether and
Diethylene glycol methyl ethyl ether have nearly the same chemical
structure (especialy with reference to the functional groups):
1. Ethylene glycol dimethyl ether: H3C-O-CH2-CH2-O-CH3
2. Diethylene glycol methyl ethyl ether: H3C-O-CH2-CH2-O-CH2-CH2-O-CH2-CH3
the same mode of interaction with living cells and tissue is expected.
Therefore, a read-across from Ethylene glycol dimethyl ether to data
obtained with Diethylene glycol methyl ethyl ether is scientifically
The present study (Vogel 2010) analyses the sensitising potential of Diethylenglycol
Three groups each of four female mice were treated daily with the
test item at concentrations of 25, 50, and 100% (w/v) in acetone:olive
oil (4+1) by topical application to the dorsum of each ear (left and
right) for three consecutive days. A control group of four mice was
treated with the vehicle (acetone:olive oil (4+1)) only. Five days after
the first topical application the mice were injected intravenously into
a tail vein with radio-labelled thymidine (3H-methyl
thymidine). Approximately five hours after intravenous injection, the
mice were sacrificed, the draining auricular lymph nodes excised and
pooled per group. Single cell suspensions of lymph node cells were
prepared from pooled lymph nodes, which were subsequently washed and
incubated with trichloroacetic acid overnight. The proliferative
capacity of pooled lymph node cells was determined by the incorporation
of3H-methyl thymidine measured in ab-scintillation counter.
All treated animals survived the scheduled study period and no
signs of toxicity were observed.
A test item is regarded as a sensitiser in the LLNA if the
exposure to one or more test concentration resulted in 3-fold or greater
increase in incorporation of3HTdR compared with concurrent
controls, as indicated by the Stimulation Index (S.I.). The estimated
concentration of test item required to produce a S.I. of 3 is referred
to as the EC3 value.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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