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EC number: 203-794-9
CAS number: 110-71-4
primary and inherent biodegradability were determined in the
Zahn-Wellens-Test / EMPA Test with a non adapted activated sludge for
the test item Monoethylenglykoldimethylether
over a period up to 48 days. The study was conducted from 2010-05-05
to OECD 302 B at Dr.U.Noack-Laboratorien.
the determination of the primary biodegradation the test item was tested
at a concentration of 600 µg/L in duplicates. The primary biodegradation
was determined by SPME GC/MS analysis of the test item. For the
determination of the inherent biodegradation the test item was tested at
a concentration of 95
in duplicates, corresponding to a DOC of 50.4
mg C/L in the test vessel. The inherent biodegradation of the test item
was followed by determination of DOC. The ratio of eliminated DOC,
corrected for the control at each time interval to the initial DOC value
is expressed as the percentage biodegradation at each sampling date.
order to check the activity of the test system diethylene glycol in a
concentration of 120 mg/L was used as functional control. After 14 days
a degradation rate of 97 % was reached.
physico-chemical elimination (volatilisation) of the test item was
monitored in separate sterile controls. At the test item concentration
of 95 mg/L
a sterile control without inoculum and poisoned with HgCl2 was
used. For determination of the primary biodegradation sterile controls
(with inoculum and poisoned with HgCl2) with a test item
concentration of 300 µg/L and 600 µg/L were tested. No physico-chemical
elimination (volatilistion) occurred in the sterile controls at 95 mg/L
until test end. At the concentration 300 µg/L and 600 µg/L the
physico-chemical elimination (volatilisation) was in the range 29 - 34%
on day 7. Until test end the mean volatilisation reached a maximum of 37
primary and inherent biodegradability of the test item in comparison to
the degradable functional control and the elimination in the sterile
controls is given in Table 1 and graphically shown in Figure 1.
the calculation of the primary biodegradation, the concentration of the
test item replicates was corrected for the mean elimination of the
sterile control values. The primary degradation started after a long
lasting adaptation phase of 39 days. The course of the biodegradation
was slow and on day 48 a biodegradation of only 14 % was reached.
inherent degradation started after a long lasting adaption phase of 26
days. The course of the biodegradation was slow and the biodegradation
did not reach the 70 % pass level. After 48 days a biodegradation of 16
% was reached.
Primary and inherent
Biodegradability of the Test Item Monoethylenglykoldimethylether in
Comparison to the Functional Control and the Sterile Control
Primary and inherent Biodegradation / Elimination [%]
95 mg/L test item
300 µg/L test item
600 µg/L test item
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