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EC number: 203-002-1
CAS number: 102-06-7
The potential of the 1,3-DIPHENYLGUANIDINE (DPG),
to induce delayed contact hypersensitivity following intradermal
injection and cutaneous application was evaluated in guinea-pigs
according to the maximization method of Magnusson and Kligman. The study
was conducted in compliance with the Principles of Good Laboratory
Fifteen guinea-pigs were allocated to two groups :
a control group of 5 females, and a treated group of 10 females. The
sensitization potential of DPG was evaluated after a 10 -day induction
period during which time the animals were treated with paraffin oil
(control group) or DPG (treated group). On day 1, in presence of
frenud's complete adjuvant, 0.1 ml of the tst substance at a
concentration of 1% (w/w) in the vehicle was administered by
intraadermal route. On day 8, 0.5 ml of DPG at a concentration of 25%
(w/w) in the vehicle was appplied by cutaneous route during 48 hours by
means of an occlusive dressing. After a period of 12 days without
treatment, a challenge cutaneous application of 0.5 ml of the vehicle
(left flank) and 0.5 ml of DPG at a concentration of 25% (w/w) in the
vehicle (right flank) were administered to all animals.
DPG and the vehicle were prepared on a dry gauze
pad then were applied to the skin and held in place for 24 hours by
means of an occlusive dressing. Cutaneous reactions on the challenge
application sites were then evaluated 24 and 48 hours after removal of
the dressing. After the final scoring period, the animals were killed.
Due to the absence of cutaneous reactions, no skin samples were taken
from the challenge application sites from all the animals.
No clinical signs and no deaths were noted during
this study. After 24 and 48 hours following removal of the dressing of
the cutaneous challenge application of the test substance, no cutaneous
reactions were recorded. The guinea-pigs showed a satisfactory
sensitization response in 100% animals using a positive sensitizer.
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