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DPG (1,3 -diphenylguanidine) has been shown to be toxic to fish and algae and harmful to daphnia in several acute studies (fish : 96 h LC50 = 4.2-11 mg/l; algae : 72 h EC50 = 7.5 mg/l; 96 h EC50 = 1.7 mg/l; daphnid : 24 h EC50 = 73.6 mg/l; 48 h EC50 = 17mg/l). The PNEC can be determined using the NOECs from the algae (0.3 mg/l), daphnid chronic 21 d (0.6 mg/l (excluding the EbC50 results)) and long-term fish (1.3 mg/L) studies, by applying an uncertainty factor of 10. The resulting PNEC would be 30 µg/l.