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EC number: 203-906-6 | CAS number: 111-77-3
Immunotoxicity
Administrative data
- Endpoint:
- immunotoxicity: acute oral
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Study period:
- 1992
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: No data on analytical purity of test substance otherwise sufficiently well reported to judge the results reliable with restrictions for use in risk assessment.
Data source
Referenceopen allclose all
- Reference Type:
- publication
- Title:
- Comparative immunosuppression of various glycol ethers orally administered to Fischer 344 rats.
- Author:
- Smialowicz RJ, Williams WC, Riddle, MM et al
- Year:
- 1 992
- Bibliographic source:
- Fundam Appl Toxicol, 18(4), 621-7
- Reference Type:
- publication
- Title:
- The immunotoxicity of 2-methoxyethanol and its metabolites.
- Author:
- Smialowicz RJ
- Year:
- 1 996
- Bibliographic source:
- Occup Hyg, vol 2, p269-74
Materials and methods
Test guideline
- Qualifier:
- no guideline followed
- Principles of method if other than guideline:
- Rats orally administered substance in two doses over a 2 day period then the humoral immune system functionality assessed with a T-dependent antigen response assay
- GLP compliance:
- not specified
Test material
- Reference substance name:
- 2-(2-methoxyethoxy)ethanol
- EC Number:
- 203-906-6
- EC Name:
- 2-(2-methoxyethoxy)ethanol
- Cas Number:
- 111-77-3
- Molecular formula:
- C5H12O3
- IUPAC Name:
- 2-(2-methoxyethoxy)ethanol
- Details on test material:
- -Source:Aldrich Chemical Company, Milwaukee, Mo
- Analytical purity:no data
Constituent 1
Test animals
- Species:
- rat
- Strain:
- Fischer 344
- Sex:
- male
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
-Source: Source: Charles River Laboratory, Raleigh, NC
-Age at study initiation: 8-10 weeks
-Housing: 3 per polycarbonate cage, heat treated pine shavings bedding.
-Diet: Purina Lab Chow, Purina Mills, ad libitum
-Water: ad libitum
ENVIRONMENTAL CONDITIONS
-Temperature: 22C
-Humidity: 50-60%
-Photoperiod: 12hrs dark/12hrs light
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- other: distilled water
- Details on exposure:
- Preparation of dosing solution: no data
- Analytical verification of doses or concentrations:
- no
- Details on analytical verification of doses or concentrations:
- dosing volume 2.5ml/kg
- Duration of treatment / exposure:
- 2 days
- Frequency of treatment:
- daily
Doses / concentrations
- Remarks:
- Doses / Concentrations:
50, 100, 200, 400mg/kg per day
Basis:
- No. of animals per sex per dose:
- 6
- Control animals:
- yes, concurrent vehicle
Examinations
- Humoral immunity examinations:
- Method: Rats were immunized with a single intravenous injection of trinitrophenyl-Lipopolysaccharide (TNP-LPS) 2-4hrs prior to substance exposure. 3 days after final exposure, a splenic antibody plaque forming assay (PFC) using SRBC was carried out using the method of Smialowicz (Toxicology 44, 271, 1987).
Dose groups: See above
Number of animals: See above. - Statistics:
- Results expressed as mean and standard deviation. Data analysed by one way analysis of variance with post analysis using Dunnett’s multiple comparison t-test or the Student-Newman-Kuels multiple range test as required. Significance level set at p<0.05.
Results and discussion
Results of examinations
- Clinical signs:
- not examined
- Mortality:
- not examined
- Body weight and weight changes:
- not examined
- Food consumption and compound intake (if feeding study):
- not examined
- Food efficiency:
- not examined
- Water consumption and compound intake (if drinking water study):
- not examined
- Ophthalmological findings:
- not examined
- Haematological findings:
- not examined
- Clinical biochemistry findings:
- not examined
- Urinalysis findings:
- not examined
- Gross pathological findings:
- not examined
- Details on results:
- Humoral immunity examinations: When expressed as cells/spleen, the 400mg/kg dose group showed a siginficant reduction but this was not evident in the 800mg/kg dose group, therefore the change in the 400mg/kg group was not regarded as biologically significant. No significant effects were seen in any of the other parameters assessed.
Specific immunotoxic examinations
- Cell viabilities:
- not examined
- Humoral immunity examinations:
- no effects observed
- Specific cell-mediated immunity:
- not examined
- Non-specific cell-mediated immunity:
- not examined
- Other functional activity assays:
- not examined
- Other findings:
- not examined
Effect levels
- Dose descriptor:
- NOAEL
- Effect level:
- > 800 mg/kg bw/day
- Sex:
- male
- Basis for effect level:
- other: Humoral immunity markers
Applicant's summary and conclusion
- Conclusions:
- Substance has no significant effects on humoral immunity.
- Executive summary:
Treatment of rats acutely with 2 -(2 -methoxyethoxy)ethanol causes no significant suppression of the antibody response to TNP-LPS at doses up to 800mg/kg.
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