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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Toxicological information

Skin irritation / corrosion

Currently viewing:

Administrative data

Endpoint:
skin irritation: in vivo
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: short report, limited documentation, pre-GLP

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1977
Report date:
1977

Materials and methods

Test guideline
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 404 (Acute Dermal Irritation / Corrosion)
GLP compliance:
no
Remarks:
pre dates GLP

Test material

Constituent 1
Chemical structure
Reference substance name:
1-chloro-2,4-dinitrobenzene
EC Number:
202-551-4
EC Name:
1-chloro-2,4-dinitrobenzene
Cas Number:
97-00-7
Molecular formula:
C6H3ClN2O4
IUPAC Name:
1-chloro-2,4-dinitrobenzene
Details on test material:
pale yellow scales

Test animals

Species:
rabbit
Strain:
Himalayan
Details on test animals or test system and environmental conditions:
animals were kept singlly and had access to food and water ad libitum.

Test system

Type of coverage:
occlusive
Preparation of test site:
other: 1 each: shaved or shaved & abraded
Vehicle:
unchanged (no vehicle)
Controls:
not required
Amount / concentration applied:
500 mg per animal
Duration of treatment / exposure:
24 h
Observation period:
72 h
Number of animals:
6
Details on study design:
The test material was applied to am area of 6,25 cm² per patch.

Results and discussion

In vivo

Resultsopen allclose all
Irritation parameter:
primary dermal irritation index (PDII)
Basis:
mean
Time point:
72 h
Score:
6
Reversibility:
not specified
Irritation parameter:
erythema score
Basis:
mean
Time point:
24/48/72 h
Score:
3
Reversibility:
not specified
Irritation parameter:
edema score
Basis:
mean
Time point:
24/48/72 h
Score:
3
Reversibility:
not specified
Other effects:
Treated area in 5 of 6 animals greyish discolored (beginning necrosis)

Applicant's summary and conclusion

Interpretation of results:
highly irritating
Remarks:
Migrated information Criteria used for interpretation of results: expert judgment
Conclusions:
1-Chlor-2,4-dinitrobenzene was highlky irritant on rabbit skin after 24 h tretment.