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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Toxicological information

Acute Toxicity: oral

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Administrative data

Endpoint:
acute toxicity: oral
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1977
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Pre-GLP; short report, limited documentation

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1977
Report date:
1977

Materials and methods

Test guideline
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 401 (Acute Oral Toxicity)
Deviations:
not specified
GLP compliance:
no
Remarks:
pre dates GLP
Test type:
standard acute method
Limit test:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
1-chloro-2,4-dinitrobenzene
EC Number:
202-551-4
EC Name:
1-chloro-2,4-dinitrobenzene
Cas Number:
97-00-7
Molecular formula:
C6H3ClN2O4
IUPAC Name:
1-chloro-2,4-dinitrobenzene
Details on test material:
slightly yelow scales

Test animals

Species:
rat
Strain:
Wistar
Sex:
male
Details on test animals or test system and environmental conditions:
Strain: WISKf (SPF 71)
Weight range: 186-226 g; mean 209 g;

Administration / exposure

Route of administration:
oral: gavage
Vehicle:
other: sesame oil
Details on oral exposure:
5% solution in sesame oil
Doses:
250-400-630-800-1000-1600 mg/kg
No. of animals per sex per dose:
10
Control animals:
no
Statistics:
determination of LD50 by probit analysis

Results and discussion

Effect levels
Sex:
male
Dose descriptor:
LD50
Effect level:
ca. 939 mg/kg bw
95% CL:
>= 816 - < 1 130
Mortality:
Dose / # of dead animals of 10
250 / 0
400 / 0
630 / 1
800 / 3
1000 / 5
1600 / 10
Clinical signs:
other: Animals died with 50 minutes to 5 days. Clincal signs in moribung animals were: prostration, reduced motoractivity, pnosis, convulsions (pre-mortal). At 1600 mg/kg additionally a sqatting posture, piloerection, increased sensitivity to noise, and tonic a
Gross pathology:
dead animals: discolored lungs, brownish blood (methemogolgin), orange discolored urine,
survivors: peritonitis with adhesions among the abdominal organs; purulent, ulcerative inflammation of the stomach lining; in some cases perforation of the stomach wall.

Applicant's summary and conclusion

Interpretation of results:
moderately toxic
Remarks:
Migrated information Criteria used for interpretation of results: expert judgment
Conclusions:
The LD 50 after oral gavage in a 5% oily solution to male rats was 939 mg/kg bw