Registration Dossier
Registration Dossier
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EC number: 202-551-4 | CAS number: 97-00-7
Acute Toxicity: oral
Administrative data
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1977
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Pre-GLP; short report, limited documentation
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 977
- Report date:
- 1977
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 401 (Acute Oral Toxicity)
- Deviations:
- not specified
- GLP compliance:
- no
- Remarks:
- pre dates GLP
- Test type:
- standard acute method
- Limit test:
- no
Test material
- Reference substance name:
- 1-chloro-2,4-dinitrobenzene
- EC Number:
- 202-551-4
- EC Name:
- 1-chloro-2,4-dinitrobenzene
- Cas Number:
- 97-00-7
- Molecular formula:
- C6H3ClN2O4
- IUPAC Name:
- 1-chloro-2,4-dinitrobenzene
- Details on test material:
- slightly yelow scales
Constituent 1
Test animals
- Species:
- rat
- Strain:
- Wistar
- Sex:
- male
- Details on test animals or test system and environmental conditions:
- Strain: WISKf (SPF 71)
Weight range: 186-226 g; mean 209 g;
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- other: sesame oil
- Details on oral exposure:
- 5% solution in sesame oil
- Doses:
- 250-400-630-800-1000-1600 mg/kg
- No. of animals per sex per dose:
- 10
- Control animals:
- no
- Statistics:
- determination of LD50 by probit analysis
Results and discussion
Effect levels
- Sex:
- male
- Dose descriptor:
- LD50
- Effect level:
- ca. 939 mg/kg bw
- 95% CL:
- >= 816 - < 1 130
- Mortality:
- Dose / # of dead animals of 10
250 / 0
400 / 0
630 / 1
800 / 3
1000 / 5
1600 / 10 - Clinical signs:
- other: Animals died with 50 minutes to 5 days. Clincal signs in moribung animals were: prostration, reduced motoractivity, pnosis, convulsions (pre-mortal). At 1600 mg/kg additionally a sqatting posture, piloerection, increased sensitivity to noise, and tonic a
- Gross pathology:
- dead animals: discolored lungs, brownish blood (methemogolgin), orange discolored urine,
survivors: peritonitis with adhesions among the abdominal organs; purulent, ulcerative inflammation of the stomach lining; in some cases perforation of the stomach wall.
Applicant's summary and conclusion
- Interpretation of results:
- moderately toxic
- Remarks:
- Migrated information Criteria used for interpretation of results: expert judgment
- Conclusions:
- The LD 50 after oral gavage in a 5% oily solution to male rats was 939 mg/kg bw
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