Registration Dossier
Registration Dossier
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EC number: 202-551-4 | CAS number: 97-00-7
Long-term toxicity to fish
Administrative data
- Endpoint:
- long-term toxicity to fish, other
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: conforms to guideline & GLP
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 994
- Report date:
- 1994
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 204 (Fish, Prolonged Toxicity Test: 14-day Study)
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- 1-chloro-2,4-dinitrobenzene
- EC Number:
- 202-551-4
- EC Name:
- 1-chloro-2,4-dinitrobenzene
- Cas Number:
- 97-00-7
- Molecular formula:
- C6H3ClN2O4
- IUPAC Name:
- 1-chloro-2,4-dinitrobenzene
- Details on test material:
- Analytical purity: 99.7 %
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- Substance concentration determined before study and once a week until the end
Test organisms
- Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
Study design
- Test type:
- flow-through
- Water media type:
- freshwater
- Total exposure duration:
- 28 d
Test conditions
- Hardness:
- 2.5 - 2.7 mmol/l
- Test temperature:
- 21 - 23 ºC
- pH:
- 7.9 - 8.3
- Details on test conditions:
- Conductivity: 630 - 700 uS/cm
Photoperiod: 6 am to 6 pm
Light intensity: approx. 700 lux
Test concentrations: 0 / 0.005 / 0.016 / 0.05 / 0.16 / 0.5 mg/l (10 fish used per concentration)
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 28 d
- Dose descriptor:
- LOEC
- Effect conc.:
- 0.16 mg/L
- Basis for effect:
- mortality
- Duration:
- 28 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.05 mg/L
- Basis for effect:
- mortality
- Duration:
- 28 d
- Dose descriptor:
- LOEC
- Effect conc.:
- 0.05 mg/L
- Basis for effect:
- other: any observed effect
- Duration:
- 28 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.016 mg/L
- Basis for effect:
- other: any observed effect
- Details on results:
- No lethality occurred in the concentration of 0.05 mg/l or less.
- Results with reference substance (positive control):
- No lethality occurred in the control group.
Applicant's summary and conclusion
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