1-chloro-2,4-dinitrobenzene

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

25.10.1994 till 01.12.1994

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Guideline conform study under GLP

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1995

Report date:

1995

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)

Deviations:

yes

Remarks:

The vessel size, the bacterial density and the concentration of test material differ

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

1-chloro-2,4-dinitrobenzene

EC Number:

202-551-4

EC Name:

1-chloro-2,4-dinitrobenzene

Cas Number:

97-00-7

Molecular formula:

C6H3ClN2O4

IUPAC Name:

1-chloro-2,4-dinitrobenzene

Details on test material:

purity: 99,7 %
Stability in water solution: 6 weeks

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

sewage, domestic, adapted

Details on inoculum:

- pre-adaptation of inoculum from municipal wastewater treatment plant Frankfurt Sindlingen
- type of inoculum: activated sludge was a centrifuged sediment washed, again centrifuged and resuspended
- pre-adapted inoculum: 12 g/l of inoculum, based on wet mud, were preadapted in the presence of 25 mg/l of test substance over 29 days with stirring and airing.

Duration of test (contact time):

28 d

Initial test substance concentration

Initial conc.:

2.87 mg/L

Based on:

test mat.

Results and discussion

% Degradation

Parameter:

% degradation (CO2 evolution)

Value:

< 20

Sampling time:

28 d

Applicant's summary and conclusion

Interpretation of results:

not inherently biodegradable