Registration Dossier
Registration Dossier
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EC number: 202-551-4 | CAS number: 97-00-7
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 25.10.1994 till 01.12.1994
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Guideline conform study under GLP
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 995
- Report date:
- 1995
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
- Deviations:
- yes
- Remarks:
- The vessel size, the bacterial density and the concentration of test material differ
- GLP compliance:
- yes
Test material
- Reference substance name:
- 1-chloro-2,4-dinitrobenzene
- EC Number:
- 202-551-4
- EC Name:
- 1-chloro-2,4-dinitrobenzene
- Cas Number:
- 97-00-7
- Molecular formula:
- C6H3ClN2O4
- IUPAC Name:
- 1-chloro-2,4-dinitrobenzene
- Details on test material:
- purity: 99,7 %
Stability in water solution: 6 weeks
Constituent 1
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- sewage, domestic, adapted
- Details on inoculum:
- - pre-adaptation of inoculum from municipal wastewater treatment plant Frankfurt Sindlingen
- type of inoculum: activated sludge was a centrifuged sediment washed, again centrifuged and resuspended
- pre-adapted inoculum: 12 g/l of inoculum, based on wet mud, were preadapted in the presence of 25 mg/l of test substance over 29 days with stirring and airing. - Duration of test (contact time):
- 28 d
Initial test substance concentration
- Initial conc.:
- 2.87 mg/L
- Based on:
- test mat.
Results and discussion
% Degradation
- Parameter:
- % degradation (CO2 evolution)
- Value:
- < 20
- Sampling time:
- 28 d
Applicant's summary and conclusion
- Interpretation of results:
- not inherently biodegradable
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