Registration Dossier
Registration Dossier
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EC number: 202-551-4 | CAS number: 97-00-7
Genetic toxicity: in vivo
Administrative data
- Endpoint:
- in vivo mammalian cell study: DNA damage and/or repair
- Remarks:
- Type of genotoxicity: DNA damage and/or repair
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Reliability:
- 2 (reliable with restrictions)
Data source
Reference
- Reference Type:
- publication
- Title:
- DNA damage induced in vivo in various tissues by nitrochlorobenzene derivatives
- Author:
- Cesarone, C.F.; Bolognesi, C.; Santi, L.
- Year:
- 1 983
- Bibliographic source:
- Mutation Research 116: 239-246 (1983)
Materials and methods
Test guideline
- Qualifier:
- no guideline available
- GLP compliance:
- not specified
- Type of assay:
- other: detection of single strand breaks by alkaline elution
Test material
- Reference substance name:
- 1-chloro-2,4-dinitrobenzene
- EC Number:
- 202-551-4
- EC Name:
- 1-chloro-2,4-dinitrobenzene
- Cas Number:
- 97-00-7
- Molecular formula:
- C6H3ClN2O4
- IUPAC Name:
- 1-chloro-2,4-dinitrobenzene
Constituent 1
Test animals
- Species:
- mouse
- Strain:
- other: Swiss CD 1
- Sex:
- male
- Details on test animals or test system and environmental conditions:
- Weight: 25 - 30 g,
Husbandry: 5-6 per cage
Temperature: 21 +/- 0.5 C
Controlled light and humidity, commercial food and water ad libitum.
Food was removed 16 h before killing.
Administration / exposure
- Route of administration:
- intraperitoneal
- Vehicle:
- DMSO
- Duration of treatment / exposure:
- 4h
- Frequency of treatment:
- single
- Post exposure period:
- not applicable
Doses / concentrations
- Remarks:
- Doses / Concentrations:
0 - 30 - 60 - 90 - 180 mg/kg bw
Basis:
no data
- No. of animals per sex per dose:
- 12
- Control animals:
- yes, concurrent vehicle
- Positive control(s):
- Nitrosomethyl urea (NMU), nitrosodimethylamine (NDMA), same procedure
Examinations
- Tissues and cell types examined:
- Brain, Liver, Kidney
- Details of tissue and slide preparation:
- Tissues were minced and cooled to 1- 4 C immediately after killing. Cellular nuclei were isolated by centrifugation in saline at 400 rpm for 2 minutes.Aliquots of about 0.5 to 1 million nuclei were transfered to membrane filters and DNA was eluted under alkaline conditions (pH = 12,3, 1 h, 6 min per fraction). DNA content was determined by a fluorimetric procedure.
Results and discussion
Test results
- Sex:
- male
- Genotoxicity:
- positive
- Remarks:
- Single strand breaks were detected in all tissues
- Toxicity:
- not specified
- Vehicle controls validity:
- valid
- Negative controls validity:
- not applicable
- Positive controls validity:
- valid
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information): positive
DNCB caused DNA single strand breaks in brains, levers, and kidney of male swiss CD1 mice.
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