Registration Dossier
Registration Dossier
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EC number: 202-551-4 | CAS number: 97-00-7
Long-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- long-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: conforms to guideline & GLP
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 994
- Report date:
- 1994
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: OECD 202. Part 2 - effects on biotic systems. 14-d reproduction test
- Deviations:
- yes
- Remarks:
- modificated to 21 days
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- 1-chloro-2,4-dinitrobenzene
- EC Number:
- 202-551-4
- EC Name:
- 1-chloro-2,4-dinitrobenzene
- Cas Number:
- 97-00-7
- Molecular formula:
- C6H3ClN2O4
- IUPAC Name:
- 1-chloro-2,4-dinitrobenzene
- Details on test material:
- Analytical purity: 99.7 %
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- water samples taken from the beginning and the end in 3 intervals
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- Clone 5 (IRChA, France):
Study design
- Test type:
- semi-static
- Water media type:
- freshwater
- Total exposure duration:
- 21 d
Test conditions
- Test temperature:
- 20.5 - 21.5 ºC
- pH:
- 8.1 - 8.4
- Dissolved oxygen:
- 8.1 - 9.0 mg/l
- Details on test conditions:
- Test concentrations: 0 / 0.01 / 0.032 / 0.1 / 0.32 / 1 mg/l (10 animals used per concentration and control group)
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 21 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.32 mg/L
- Basis for effect:
- immobilisation
- Duration:
- 21 d
- Dose descriptor:
- LOEC
- Effect conc.:
- 1 mg/L
- Basis for effect:
- immobilisation
- Duration:
- 21 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.1 mg/L
- Basis for effect:
- reproduction
- Duration:
- 21 d
- Dose descriptor:
- LOEC
- Effect conc.:
- 0.32 mg/L
- Basis for effect:
- reproduction
Applicant's summary and conclusion
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