Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 235-120-4
CAS number: 12070-08-5
The skin sensitising properties of titanium
carbide were tested in an in vivo local lymph node assay (OECD
429), in which test item did not show skin sensitising effects.
No effects were observed on the body weights
and the lymph node weights of the animals tested with titanium carbide.
No other signs of toxicity were seen in any of the animals.
Table 1: Mean weight of lymph nodes.
Mean of test group
12.5% TiC in vehicle
25% TiC in vehicle
50% TiC in vehicle
In this dermal sensitization study with
titanium carbide in acetone/olive oil 4:1 (v/v), young adult female
CBA/CaOlaHsD mice were tested using the Local Lymph Node Assay (LLNA).
25 µl of the test substance in solvent were applied on the entire
dorsal ear surface of each animal. The following concentrations were
used: 12.5, 25 and 50%. Acetone/olive oil 4:1 was used as negative
control. Phenylenediamine was tested as a positive control in a prior
assay. The application was repeated daily for three consecutive days.
The doses applied were selected based on a pre-screening assay.
Five days after the 1st application 250 µL 20 µCi 3H-methyl
thymidine was injected intravenously to all mice (diluted
concentration: 80 µCi/mL). All animals were sacrificed five hours
after the aforementioned injection. The draining ear lymph nodes were
excised into PBS and single cell suspension of lymphocyte was
isolated. Cells were precipitated with 5% trichloroacetic acid at 4 °C
for 18 hours.The radioactivity was measured in a ß-counter.
Stimulation indices (ratio of 3H-methyl
thymidine incorporation into lymph node of test animals relative to
that for control animals)≥ 3 were considered a positive response. The
results revealed the following SI: 1.8, 1.7 and 1.9 for the
concentrations of 12.5, 25 and 50%, respectively. No signs of toxicity
were detected in any of the treated animals. In this study,
Titanimum carbide is not a dermal sensitizer.
carbide in acetone/olive oil 4:1 (v/v) was tested in 5 young adult
females per dose according to OECD 429 (LLNA). 25 µl of the test
substance in solvent were applied on the entire dorsal ear surface of
each animal at a concentration of 12.5, 25 and 50%. Phenylenediamine was
tested as a positive control.
indices (SI) (ratio of 3H-methyl thymidine incorporation into lymph node
of test animals relative to that for control animals) ≥ 3 were
considered a positive response. The results for the test item revealed
SIs of 1.8, 1.7 and 1.9 for 12.5, 25 and 50%, respectively. No signs of
toxicity were detected in any of the treated animals. In this study,
Titanium carbide is not a dermal sensitizer.
on the results of a valid OECD 429 assay (LLNA), titanium carbide is not
a dermal sensitizer and classification according to Regulation (EC) No.
1272/2008 is not warranted.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
Sellel veebilehel kasutatakse küpsiseid, et tagada lehe parim kasutus.
Welcome to the ECHA website. This site is not fully supported in Internet Explorer 7 (and earlier versions). Please upgrade your Internet Explorer to a newer version.
Do not show this message again