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EC number: 235-120-4
CAS number: 12070-08-5
No effects were observed on the body weights and the lymph node weights
of the animals tested with titanium carbide. No other signs of toxicity
were seen in any of the animals.
Table 1: Mean weight of lymph nodes.
Mean of test group
12.5% TiC in vehicle
25% TiC in vehicle
50% TiC in vehicle
In this dermal sensitization study with titanium
carbide in acetone/olive oil 4:1 (v/v), young adult female
CBA/CaOlaHsD mice were tested using the Local Lymph Node Assay (LLNA).
25 µl of the test substance in solvent were applied on the entire
dorsal ear surface of each animal. The following concentrations were
used: 12.5, 25 and 50%. Acetone/olive oil 4:1 was used as negative
control. Phenylenediamine was tested as a positive control in a prior
assay. The application was repeated daily for three consecutive days.
The doses applied were selected based on a pre-screening assay.
Five days after the 1st application 250 µL 20 µCi 3H-methyl
thymidine was injected intravenously to all mice (diluted
concentration: 80 µCi/mL). All animals were sacrificed five hours
after the aforementioned injection. The draining ear lymph nodes were
excised into PBS and single cell suspension of lymphocyte was
isolated. Cells were precipitated with 5% trichloroacetic acid at 4 °C
for 18 hours.The radioactivity was measured in a ß-counter.
Stimulation indices (ratio of 3H-methyl thymidine
incorporation into lymph node of test animals relative to that for
control animals)≥ 3 were considered a positive response. The results
revealed the following SI: 1.8, 1.7 and 1.9 for the concentrations of
12.5, 25 and 50%, respectively. No signs of toxicity were detected in
any of the treated animals. In this study, Titanimum carbide is
not a dermal sensitizer.
Titanium carbide in acetone/olive oil
4:1 (v/v) was tested in 5 young adult females per dose according to OECD
429 (LLNA). 25 µl of the test substance in solvent were applied on the
entire dorsal ear surface of each animal at a concentration of 12.5, 25
and 50%. Phenylenediamine was tested as a positive control.
Stimulation indices (SI) (ratio of
3H-methyl thymidine incorporation into lymph node of test animals
relative to that for control animals) ≥ 3 were considered a positive
response. The results revealed SIs of 1.8, 1.7 and 1.9 for 12.5, 25 and
50%, respectively. No signs of toxicity were detected in any of the
treated animals. In this study, Titanium carbide is not a dermal
carbide is not a dermal sensitizer in a valid OECD 429 assay (LLNA).
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