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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Toxicological information

Genetic toxicity: in vitro

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Administrative data

in vitro gene mutation study in bacteria
Type of genotoxicity: gene mutation
Type of information:
migrated information: read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
supporting study
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: comparable to guidelines, comprehensive evaluation programme

Data source

Reference Type:
Salmonella Mutagenicity Tests: V. Results from the Testing of 311 Chemicals.
Zeiger, E., Anderson, B., Haworth, S., Lawlor, T., Mortelmans, K.
Bibliographic source:
Environ. Molec. Mutagen. 19 (Suppl.21), 2-141

Materials and methods

Test guideline
equivalent or similar to guideline
OECD Guideline 471 (Bacterial Reverse Mutation Assay)
GLP compliance:
not specified
Type of assay:
bacterial reverse mutation assay

Test material

Constituent 1
Chemical structure
Reference substance name:
trisodium 2-[bis(carboxylatomethyl)amino]acetate hydrate
EC Number:
Cas Number:
Molecular formula:
trisodium 2-[bis(carboxylatomethyl)amino]acetate hydrate
Details on test material:
Na3.NTA.H2O, commercial, purity 89%


Target gene:
S. typhimurium
Species / strain
Species / strain / cell type:
other: Salmonella typhimurium TA100, 98, 1535, 1537, 97
Metabolic activation:
with and without
Metabolic activation system:
rat liver S-9 mix
Test concentrations with justification for top dose:
0, 33 - 2000 ug/plate
Vehicle / solvent:
The solvent of choice was distilled water, followed by dimethyl sulfoxide (DMSO), 95% ethanol, and acetone.
Details on test system and experimental conditions:
Salmonella typhimurium strains were obtained from Dr . Bruce Ames (University of California, Berkeley) and stored as recommended [Maron and Ames,
19831. Cultures were grown at 37°C overnight, with shaking, in Oxoid #2 broth (CWR, MIC), or in defined minimal medium supplemented with biotin (0.8 ug/ml) and histidine (40 ug/ml) (SRI). The phenotypes of the strains were analyzed at the time of their use in mutagenicity assays.
The S-9 (9,000 X g supernatant) fractions of Aroclor 1254-induced, male Sprague-Dawley rat and male Syrian hamster livers were prepared as described previously [Haworth et al ., 1983]. The S-9 mixes were prepared immediately prior to use and contained either 10% or 30% S-9; occasionally, 5% S-9 was also used. All chemicals were tested in the absence of metabolic activation, and with rat and hamster S-9 fractions.

Results and discussion

Test results
Metabolic activation:
with and without
Remarks on result:
other: other: Salmonella typhimurium TA100, 98, 1535, 1537, 97
Migrated from field 'Test system'.

Applicant's summary and conclusion

Interpretation of results (migrated information):