Registration Dossier
Registration Dossier
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 225-768-6 | CAS number: 5064-31-3
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- migrated information: read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- supporting study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: comparable to guidelines, comprehensive evaluation programme
Data source
Reference
- Reference Type:
- publication
- Title:
- Salmonella Mutagenicity Tests: V. Results from the Testing of 311 Chemicals.
- Author:
- Zeiger, E., Anderson, B., Haworth, S., Lawlor, T., Mortelmans, K.
- Year:
- 1 992
- Bibliographic source:
- Environ. Molec. Mutagen. 19 (Suppl.21), 2-141
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- GLP compliance:
- not specified
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- trisodium 2-[bis(carboxylatomethyl)amino]acetate hydrate
- EC Number:
- 606-091-9
- Cas Number:
- 18662-53-8
- Molecular formula:
- C6H8NNa3O7
- IUPAC Name:
- trisodium 2-[bis(carboxylatomethyl)amino]acetate hydrate
- Details on test material:
- Na3.NTA.H2O, commercial, purity 89%
Constituent 1
Method
- Target gene:
- S. typhimurium
Species / strain
- Species / strain / cell type:
- other: Salmonella typhimurium TA100, 98, 1535, 1537, 97
- Metabolic activation:
- with and without
- Metabolic activation system:
- rat liver S-9 mix
- Test concentrations with justification for top dose:
- 0, 33 - 2000 ug/plate
- Vehicle / solvent:
- The solvent of choice was distilled water, followed by dimethyl sulfoxide (DMSO), 95% ethanol, and acetone.
- Details on test system and experimental conditions:
- Salmonella typhimurium strains were obtained from Dr . Bruce Ames (University of California, Berkeley) and stored as recommended [Maron and Ames,
19831. Cultures were grown at 37°C overnight, with shaking, in Oxoid #2 broth (CWR, MIC), or in defined minimal medium supplemented with biotin (0.8 ug/ml) and histidine (40 ug/ml) (SRI). The phenotypes of the strains were analyzed at the time of their use in mutagenicity assays.
The S-9 (9,000 X g supernatant) fractions of Aroclor 1254-induced, male Sprague-Dawley rat and male Syrian hamster livers were prepared as described previously [Haworth et al ., 1983]. The S-9 mixes were prepared immediately prior to use and contained either 10% or 30% S-9; occasionally, 5% S-9 was also used. All chemicals were tested in the absence of metabolic activation, and with rat and hamster S-9 fractions.
Results and discussion
Test results
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Remarks on result:
- other: other: Salmonella typhimurium TA100, 98, 1535, 1537, 97
- Remarks:
- Migrated from field 'Test system'.
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
negative
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
