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In accordance with column 2 of REACH Annex IX, trisodium nitrilotriacetate (Na3NTA) has a log octanol-water partition coefficient of -13.2 at pH 7, is highly water-soluble, and is unlikely, due to its polar nature, to be taken up by fish gills or across other biological membranes. Therefore, there is no need to newly perform a reliable (fish) bioaccumulation study according to current guidelines, especially as secondary references are available which indicate that only a low accumulation of Na3NTA occurs in the hydrosphere.