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Toxicity to microorganisms

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Description of key information

The 3-hour NOEC of FAT 40825/A to activated sludge microorganisms is at least 1000 mg/L. The 3-hour EC20, EC50 and EC80 could not be calculated, but are clearly higher than 1000 mg/L.

Key value for chemical safety assessment

EC50 for microorganisms:
1 000 mg/L
EC10 or NOEC for microorganisms:
1 000 mg/L

Additional information

The inhibitory effect of the test item FAT 40825/A on the respiration rate of aerobic wastewater microorganisms of activated sludge was investigated in a 3-hour respiration inhibition test according to the OECD Guideline for Testing of Chemicals, No. 209.

A limit test with a nominal test concentration of 1000 mg/L was performed to demonstrate that the test item has no toxic effect on activated sludge microorganisms up to and including this concentration.

In addition, two controls and three different concentrations of the reference item 3,5-dichlorophenol (5, 16, and 50 mg/L) were tested in parallel. The results of these treatments confirmed suitability of the activated sludge and the method used.

The test item FAT 40825/A had no significant inhibitory effect (< 15 %) on the respiration rate of activated sludge after the incubation period of three hours at the limit test concentration of 1000 mg/L. Thus, the 3-hour NOEC of FAT 40825/A to activated sludge microorganisms is at least 1000 mg/L. The 3-hour EC20, EC50 and EC80 could not be calculated, but are clearly higher than 1000 mg/L.