Tetrasodium [2-({4-fluoro-6-[(2-{[4-fluoro-6-({5-(hydroxykappaO)-6-[(2-{[2-(hydroxy-kappaO)-5-sulfophenyl] diazenyl-kappaN1}-4,5-dimethoxyphenyl) diazenyl-kappaN2]-7-sulfo-2-naphthyl} amino)-1,3,5-triazin-2-yl] amino} propyl) amino]-1,3,5-triazin-2-yl} amino) benzene-1,4- disulfonato(6-)] cuprate(4-)

Water solubility

Link to relevant study record(s)

Reference

Endpoint:

water solubility

Type of information:

experimental study

Adequacy of study:

key study

Study period:

Experiment start date - 31 May 2006; Experiment completion date - 03 June 2006; Study completion - 20 July 2006

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 105 (Water Solubility)

Deviations:

no

Qualifier:

according to guideline

Guideline:

other: EEC Directive 92/69, Part A, Methods for the determination of physico-chemical properties, A.6: "Water solubility", EEC Publication No. L383, December 1992.

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Type of method:

flask method

Specific details on test material used for the study:

Identity: FAT 40825/A
Batch number: CHU 297 / BOP 04/05
Purity: Organic part (Na-salt): approx. 83.7 %; All coloured components: approx. 80.54 %; Main component: approx. 59.1 %.
Appearance: Solid, black powder
Storage conditions: At room temperature at about 20 °C
Expiration date: December 31, 2010

Water solubility:

>= 341 g/L

Conc. based on:

test mat.

Temp.:

22 °C

Remarks on result:

other: pH not specified

Conclusions:

The water solubility of FAT 40825/A was found to be at least 341 g/l at room temperature. The test item was found to be very soluble (>10.0 g/L)

Executive summary:

The determination of the water solubility of FAT 40825/A was performed according to the EEC Directive 92/69, A.6 "Water Solubility" (1992) and the OECD Guideline No. 105 "Water Solubility" (1995) using a simplified flask method. After mixing a total of about 2 g of test item in 5 ml of water, a dark brown, viscous solution was obtained. It was scarily possible to decide by visual judgment if complete dissolution was achieved. The water solubility of FAT 40825/A might therefore be higher as determined in the present study. The determination of the concentration in the above-mentioned solution was confirmed by HPLC with respect to a calibration curve. A main test using the flask shaking method was not possible as no saturated solution of test item in water can be prepared. Due to the results in the preliminary test, the water solubility of FAT 40825/A was found to be at least 341 g/l at room temperature.

Description of key information

The water solubility of FAT 40825/A was found to be at least 341 g/l at room temperature.

Key value for chemical safety assessment

Water solubility:

341 g/L

at the temperature of:

20 °C

Additional information

The determination of the water solubility of FAT 40825/A was performed according to the EEC Directive 92/69, A.6 "Water Solubility" (1992) and the OECD Guideline No. 105 "Water Solubility" (1995) using a simplified flask method. After mixing a total of about 2 g of test item in 5 ml of water, a dark brown, viscous solution was obtained. It was scarily possible to decide by visual judgment if complete dissolution was achieved. The water solubility of FAT 40825/A might therefore be higher as determined in the present study. The determination of the concentration in the above-mentioned solution was confirmed by HPLC with respect to a calibration curve. A main test using the flask shaking method was not possible as no saturated solution of test item in water can be prepared. Due to the results in the preliminary test, the water solubility of FAT 40825/A was found to be at least 341 g/l at room temperature.