Registration Dossier

Ecotoxicological information

Short-term toxicity to fish

Currently viewing:

Administrative data

Link to relevant study record(s)

Reference
Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1999
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study with acceptable restrictions
Remarks:
Althought the study methodology followed OECD TG 203, EPA OTS 797.1400 and ASTM guidelines, it is unclear whether the study was conducted as per Good Laboratory Practices (GLP) and the report contains no information on analytical measurements of the tested doses.
Qualifier:
according to
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
Deviations:
not specified
Qualifier:
according to
Guideline:
EPA OTS 797.1400 (Fish Acute Toxicity Test)
Deviations:
not specified
Qualifier:
according to
Guideline:
other: Annual Book of ASTM Standards, Water and Environmental Technology, Vol. 11.05, (1995)
Deviations:
not specified
Principles of method if other than guideline:
not applicable
GLP compliance:
not specified
Specific details on test material used for the study:
- Name of test material (as cited in study report): Hexyl Carbitol Solvent
- Physical state: liquid
Analytical monitoring:
no
Vehicle:
no
Details on test solutions:
Dechlorinated Charleston municipal water was used in tests as dilution water and in experimental controls and the same water was used for maintaining the in-house fathead minnow stock. The incoming water was treated with activated carbon in a two-gallon filter yielding a chlorine-free dilution water with a pH very close to 7.0. The carbon beds were changed as needed to maintain water quality.
Test organisms (species):
Pimephales sp.
Details on test organisms:
TEST ORGANISM
- Common name: fathead minnows
- Strain: no information available
- Source: in-house stock (UCC laboratory culturing program)
- Age at study initiation (mean and range, SD): 21-60 days
- Length at study initiation (length definition, mean, range and SD): average fish length ranged from 10-15 mm
- Weight at study initiation (mean and range, SD): average wet weight/fish ranged from 10-25 grams
- Other: organism loading ranged from 0.13-0.33 g wet-weight fish/dose
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h
Hardness:
40 to 60 mg/L as CaCO3 (measured)
Test temperature:
no information available
pH:
close to 7.0
Dissolved oxygen:
no information available
Nominal and measured concentrations:
- Nominal concentrations: no information available.
Details on test conditions:
A minimum of 5 concentrations of each test substance were utilized, with each 1.5 liter volume containing 20 fish fry. Fish survival and physical response, as well as temperature, pH and dissolved oxygen levels, were monitored daily during the test period of 96 hours.
Reference substance (positive control):
not required
Key result
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
200 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: 95% CL -180-210 mg/L
Reported statistics and error estimates:
The concentration of test product which affected 50% of the test population was calculated for the 96-hour exposure period by the Spearman-Karber estimator. The 95% confidence intervals were also provided by this program.

ADDITIONAL REPORTED EFFECT CONCENTRATIONS

96-h NOEC = 95 mg/L

Description of key information

DGHE is with high probability acutely not harmful to fish.

The 96-h LC50 value is 200 mg/L in freshwater fish (Pimephales sp.)

Key value for chemical safety assessment

Additional information

The short-term toxicity to freshwater fish was determined in a study according to OECD TG 203. In this study, groups of 20 Pimephales sp. were exposed for 96 hours to five concentrations of test substance under static conditions. No analytical verification of the test material performed. The 96-hour LC50 of the test material was calculated to be 200 mg/L (95% fiducial limits ranging between 180-210 mg/L).