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Ecotoxicological information

Long-term toxicity to aquatic invertebrates

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Reference
Endpoint:
long-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
study well documented, meets generally accepted scientific principles, acceptable for assessment
Qualifier:
according to
Guideline:
other: AFNOR NF T 90-376
GLP compliance:
not specified
Specific details on test material used for the study:
- Name of test material (as cited in study report): Diethylene glycol monohexyl ether
- Analytical purity: 95 %
Analytical monitoring:
no
Vehicle:
not specified
Test organisms (species):
Ceriodaphnia dubia
Details on test organisms:
TEST ORGANISM
- Age: Young organisms (< 24 hours old) and from the second to fifth brood of healthy adults reared in the laboratory
- Feeding during test: Chorella vulgaris (6 x 10E6 cells/50 mL), Raphidocelis subcapitata (3 x 10E6 cells/50 mL) and 25 microliters/50 mL of a stock solution (5 g/L) of commercial fish food.

Test type:
semi-static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
7 d
Hardness:
250 ± 20 mg/L CaCO3
Test temperature:
25 ± 2 °C
pH:
8 ± 0.3
Dissolved oxygen:
8.6 - 9.2 mg/L
Nominal and measured concentrations:
- Nominal concentrations: A range of 5 concentrations spanning those causing 0-100% inhibition of reproduction were used. Not further specified.
Details on test conditions:
TEST SYSTEM
- Material, total volume, fill volume: glass vials, 100 mL, 50 mL
- Renewal rate of test solution (frequency/flow rate): daily (with the exception of weekends)
- No. of organisms per vessel: 1
- No. of vessels per concentration (replicates): 10
- No. of vessels per control (replicates): 10

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Evian mineral water
- Intervals of water quality measurement: Hardness, pH, oxygen content daily (except weekends), temperature continuously

OTHER TEST CONDITIONS
- Photoperiod: 16 h : 8 h light : darkness
- Light intensity: at the air/water interface ranged between 300 and 500 lx

EFFECT PARAMETERS MEASURED:
- Reproduction after 7 days


Reference substance (positive control):
yes
Remarks:
sodium pentachlorophenate; copper (II) sulfate pentahydrate
Key result
Duration:
7 d
Dose descriptor:
EC10
Effect conc.:
19.63 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
reproduction
Remarks on result:
other: Confindence interval: 5.8 - 45.61 mg/L
Reported statistics and error estimates:
The EC10 value was calculated according to the method of Hill (Garrie et al., Water Res. 24:59-65, 1990 and Vindimian et al., J Appl Biochem 5:261-268, 1983).

Description of key information

The 7-day EC10 value is 19.63 mg/L in aquatic invertebrates Ceriodaphnia dubia.

Key value for chemical safety assessment

Additional information

The chronic toxicity to aquatic invertebrates was determined in a non-GLP study according to AFNOR NF T90-376. In this study daphnids (Ceriodaphnia dubia, 1 per vessel, 10 replicates) were exposed to A range of 5 concentrations spanning those causing 0-100% inhibition of reproduction for 7 days under semi-static conditions (test medium renewal each 24 hours, with the exception of weekends). The 7-d EC10 value was determined at 19.63 mg/L.