2-(2-hexyloxyethoxy)ethanol

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

yes

Specific details on test material used for the study:

- Name of test material (as cited in study report): N-hexyldiglykol roh
- Physical state: colourless, fluid
- Analytical purity: not reported
- Batch No.: from continuous production

Analytical monitoring:

yes

Vehicle:

yes

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: The test substance was stirred in M4 medium for about 10 minutes at 20 ± 2°C. The nominal concentration of the stock solution was 100 mg/L. By diluting this stock solution with M4 medium the nominal concentrations were prepared.

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: water flea
- Source: Originally from "Institut National de Recherche Chimique appliquee, France", and has been bred from 1978 in the laboratories of BASF
- Age at study initiation: 2-24 h
- Feeding during test: none

ACCLIMATION
- Acclimation period: none

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Hardness:

2.20 - 3.20 mmol/ L

Test temperature:

19.5 - 20.9 °C

pH:

8.0 - 8.1

Dissolved oxygen:

8.3 - 8.7 mg/L

Nominal and measured concentrations:

- Nominal test concentrations: 0, 12.5, 25, 50, 100 mg/L
- Analytical concentrations: < 5, 13, n.m., n.m., 94 mg/L
n.m = not measured

Details on test conditions:

TEST SYSTEM
- Test vessel: Test tubes (glass) with flat bottom (nominal volume 20 mL)
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- Biomass loading rate: 0.5

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: A synthetic fresh water was used for culture and test purposes. This water was prepared on the basis of an
ultrapure, demineralized water with a conductivity < 0 .05 µS/cm. For the composition of this M4 medium see ISO 10706.
- Alkalinity: 0.80 - 1.00 mmol/L
- Ca/Mg ratio: 4:1
- Conductivity: 650 µS/cm
- Intervals of water quality measurement: once at start of experiment

OTHER TEST CONDITIONS
- Photoperiod: 16:8 day-night regime
- Light intensity: About 2- 7 µE/(m²*s) at a wave length of 400 - 700 nm

EFFECT PARAMETERS MEASURED:
- mobility after 0, 3, 6, 24 and 48 h

Reference substance (positive control):

no

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

ADDITIONAL REPORTED EFFECT CONCENTRATIONS

- 48 -h EC0: ≥100 mg/L

- 48 -h EC100: >100 mg/L

Description of key information

DGHE is with high probability acutely not harmful to aquatic invertebrates.

The 48-h EC50 value is >100 mg/L in aquatic invertebrates Daphnia magna.

Additional information

The acute toxicity to aquatic invertebrates was determined in a study according to OECD TG 202 and in compliance with GLP criteria (BASFSE, 1999). In this study daphnids (D. magna, 5 per concentration) were exposed to nominal concentrations of 0, 12.5, 25, 50, 100 mg/L for 48 hours. The two lowest and the highest concentration were analytically verified and remained within ±20%. Therefore, effect concentrations are expressed as nominal. Immobilization was recorded after 48 hours exposure. No immobilization was observed in any of the test concentrations. Based on these findings the 48 -h EC50 value was determined at >100.0 mg/L.

This result is supported by another study performed also according to OECD TG 202 (UCC/Dow, 1999). In this study daphnids (D. magna, 5 per concentration) were exposed to an unspecified range of test concentrations for 48 hours under static conditions. Immobilization was recorded after 48 hours exposure. The 48-h LC50 in this study was determined at 370 mg/L (95% C.I. 330 - 420 mg/L).