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Short-term toxicity to aquatic invertebrates

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Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
GLP compliance:
yes
Specific details on test material used for the study:
- Name of test material (as cited in study report): N-hexyldiglykol roh
- Physical state: colourless, fluid
- Analytical purity: not reported
- Batch No.: from continuous production
Analytical monitoring:
yes
Vehicle:
yes
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: The test substance was stirred in M4 medium for about 10 minutes at 20 ± 2°C. The nominal concentration of the stock solution was 100 mg/L. By diluting this stock solution with M4 medium the nominal concentrations were prepared.
Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Common name: water flea
- Source: Originally from "Institut National de Recherche Chimique appliquee, France", and has been bred from 1978 in the laboratories of BASF
- Age at study initiation: 2-24 h
- Feeding during test: none

ACCLIMATION
- Acclimation period: none
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h
Hardness:
2.20 - 3.20 mmol/ L
Test temperature:
19.5 - 20.9 °C
pH:
8.0 - 8.1
Dissolved oxygen:
8.3 - 8.7 mg/L
Nominal and measured concentrations:
- Nominal test concentrations: 0, 12.5, 25, 50, 100 mg/L
- Analytical concentrations: < 5, 13, n.m., n.m., 94 mg/L
n.m = not measured
Details on test conditions:
TEST SYSTEM
- Test vessel: Test tubes (glass) with flat bottom (nominal volume 20 mL)
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- Biomass loading rate: 0.5

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: A synthetic fresh water was used for culture and test purposes. This water was prepared on the basis of an
ultrapure, demineralized water with a conductivity < 0 .05 µS/cm. For the composition of this M4 medium see ISO 10706.
- Alkalinity: 0.80 - 1.00 mmol/L
- Ca/Mg ratio: 4:1
- Conductivity: 650 µS/cm
- Intervals of water quality measurement: once at start of experiment

OTHER TEST CONDITIONS
- Photoperiod: 16:8 day-night regime
- Light intensity: About 2- 7 µE/(m²*s) at a wave length of 400 - 700 nm

EFFECT PARAMETERS MEASURED:
- mobility after 0, 3, 6, 24 and 48 h
Reference substance (positive control):
no
Key result
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility

ADDITIONAL REPORTED EFFECT CONCENTRATIONS

- 48 -h EC0: ≥100 mg/L

- 48 -h EC100: >100 mg/L

Description of key information

DGHE is with high probability acutely not harmful to aquatic invertebrates.

The 48-h EC50 value is >100 mg/L in aquatic invertebrates Daphnia magna.

Key value for chemical safety assessment

Additional information

The acute toxicity to aquatic invertebrates was determined in a study according to OECD TG 202 and in compliance with GLP criteria (BASFSE, 1999). In this study daphnids (D. magna, 5 per concentration) were exposed to nominal concentrations of 0, 12.5, 25, 50, 100 mg/L for 48 hours. The two lowest and the highest concentration were analytically verified and remained within ±20%. Therefore, effect concentrations are expressed as nominal. Immobilization was recorded after 48 hours exposure. No immobilization was observed in any of the test concentrations. Based on these findings the 48 -h EC50 value was determined at >100.0 mg/L.

This result is supported by another study performed also according to OECD TG 202 (UCC/Dow, 1999). In this study daphnids (D. magna, 5 per concentration) were exposed to an unspecified range of test concentrations for 48 hours under static conditions. Immobilization was recorded after 48 hours exposure. The 48-h LC50 in this study was determined at 370 mg/L (95% C.I. 330 - 420 mg/L).